BRENDA - Enzyme Database
show all sequences of 2.4.1.185

cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10)

Noguchi, A.; Sasaki, N.; Nakao, M.; Fukami, H.; Takahashi, S.; Nishino, T.; Nakayama, T.; J. Mol. Catal. B 55, 84-92 (2008)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Fe2+
enzyme activity is partially inhibited by 0.1 mM Fe2+ (43% residual activity)
Lycium barbarum
Hg2+
enzyme activity is completely inhibited by 0.1 mM Hg2+
Lycium barbarum
additional information
Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+, and Zn2+ (0.1 mM each) have negligible effects on catalytic activity
Lycium barbarum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0093
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lycium barbarum
B6EWX3
Chinese wolfberry
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Lycium barbarum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor
705189
Lycium barbarum
?
-
-
-
-
UDP-glucose + naringenin
enzyme specificity for UDP-glucose
705189
Lycium barbarum
UDP + naringenin 7-O-glucoside
-
-
-
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable below 40C, pH 7.2, for 20 min
Lycium barbarum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.89
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
at 30C
Lycium barbarum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Lycium barbarum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
at 20C for 60 min
Lycium barbarum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Fe2+
enzyme activity is partially inhibited by 0.1 mM Fe2+ (43% residual activity)
Lycium barbarum
Hg2+
enzyme activity is completely inhibited by 0.1 mM Hg2+
Lycium barbarum
additional information
Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+, and Zn2+ (0.1 mM each) have negligible effects on catalytic activity
Lycium barbarum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0093
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Lycium barbarum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor
705189
Lycium barbarum
?
-
-
-
-
UDP-glucose + naringenin
enzyme specificity for UDP-glucose
705189
Lycium barbarum
UDP + naringenin 7-O-glucoside
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable below 40C, pH 7.2, for 20 min
Lycium barbarum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.89
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
at 30C
Lycium barbarum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Lycium barbarum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
at 20C for 60 min
Lycium barbarum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
97
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
97
-
naringenin
at pH 7.2 and 30C
Lycium barbarum
Other publictions for EC 2.4.1.185
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705189
Noguchi
-
cDNA cloning of glycosyltransf ...
Lycium barbarum
J. Mol. Catal. B
55
84-92
2008
-
-
-
-
-
-
3
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
1
1
488819
Durren
Flavanone-7-O-glucosyltransfer ...
Petunia x hybrida
Phytochemistry
52
793-798
1999
-
-
-
-
-
-
1
5
-
1
1
1
-
1
-
-
1
-
-
3
-
-
7
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
5
-
1
1
1
-
-
-
1
-
3
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488816
McIntosh
Flavanone-specific 7-O-glucosy ...
Citrus x paradisi
Arch. Biochem. Biophys.
282
50-57
1990
-
-
-
-
-
-
-
3
-
-
1
2
-
1
-
-
1
-
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
2
-
-
-
1
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
488817
McIntosh
-
Biosynthesis of naringin in Ci ...
Citrus x paradisi
Phytochemistry
29
1533-1538
1990
-
-
-
-
-
-
6
4
-
1
-
1
-
1
-
-
1
-
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
-
1
-
1
-
-
-
1
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488818
Lewinsohn
Flavanone glycoside biosynthes ...
Citrus maxima, Citrus mitis
Plant Physiol.
91
1323-1328
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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