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Literature summary for 2.4.1.185 extracted from

  • Noguchi, A.; Sasaki, N.; Nakao, M.; Fukami, H.; Takahashi, S.; Nishino, T.; Nakayama, T.
    cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10) (2008), J. Mol. Catal. B, 55, 84-92.
No PubMed abstract available
Search for more literature for 2.4.1.185

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ enzyme activity is partially inhibited by 0.1 mM Fe2+ (43% residual activity) Lycium barbarum
Hg2+ enzyme activity is completely inhibited by 0.1 mM Hg2+ Lycium barbarum
additional information Ca2+, Cd2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Sn2+, and Zn2+ (0.1 mM each) have negligible effects on catalytic activity Lycium barbarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
 naringenin at pH 7.2 and 30°C Lycium barbarum

Organism

Organism UniProt Comment Textmining
Lycium barbarum B6EWX3 Chinese wolfberry
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Lycium barbarum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor Lycium barbarum ?
-
 ?
UDP-glucose + naringenin enzyme specificity for UDP-glucose Lycium barbarum UDP + naringenin 7-O-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
UGT73A10 a flavonoid 7-O-glucosyltransferase with broad acceptor specificity Lycium barbarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable below 40°C, pH 7.2, for 20 min Lycium barbarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.89
-
 naringenin at pH 7.2 and 30°C Lycium barbarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 at 30°C Lycium barbarum

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
 Lycium barbarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 at 20°C for 60 min Lycium barbarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
97
-
 naringenin at pH 7.2 and 30°C Lycium barbarum