BRENDA - Enzyme Database
show all sequences of 2.4.1.185

Biosynthesis of naringin in Citrus paradisi: UDP-glucosyl-transferase activity in grapefruit seedlings

McIntosh, C.A.; Mansell R.L.; Phytochemistry 29, 1533-1538 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
10 mM, strong
Citrus x paradisi
ATP
10 mM, strong
Citrus x paradisi
NADPH
10 mM, weak
Citrus x paradisi
TTP
10 mM, strong
Citrus x paradisi
UDP
10 mM, weak
Citrus x paradisi
UTP
10 mM, strong
Citrus x paradisi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
naringenin
-
Citrus x paradisi
0.11
-
hesperetin
-
Citrus x paradisi
0.13
-
2',4',6',4-tetrahydroxy chalcone
-
Citrus x paradisi
0.37
-
UDPglucose
-
Citrus x paradisi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
CaCl2, MnCl2, NaCl, MgCl2 at 0.1 mM, 1.0 mM and 10 mM have no effect
Citrus x paradisi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
trivial name naringenin
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
-
Citrus x paradisi
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus x paradisi
-
-
-
Purification (Commentary)
Commentary
Organism
-
Citrus x paradisi
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
highest activity in leaves, more than in stem and roots
Citrus x paradisi
-
Storage Stability
Storage Stability
Organism
4°C, 1% bovine serum albumin, 2-3 days, no loss of activity
Citrus x paradisi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + hesperetin
-
488817
Citrus x paradisi
UDP + hesperetin 7-O-glucoside
-
488817
Citrus x paradisi
?
UDP-glucose + naringenin
-
488817
Citrus x paradisi
UDP + naringenin 7-O-glucoside
-
-
-
-
UDPglucose + 2',4',6',4-tetrahydroxychalcone
64% of activity with naringenin
488817
Citrus x paradisi
UDP + 2',4',6',4-tetrahydroxychalcone 4'-O-glucoside
-
488817
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
trivial name naringenin
488817
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
-
488817
Citrus x paradisi
-
UDPglucose + kaempferol
90% of activity with naringenin
488817
Citrus x paradisi
UDP + kaempferol 7-O-glucoside
-
488817
Citrus x paradisi
?
UDPglucose + quercetin
164% of activity with naringenin
488817
Citrus x paradisi
UDP + quercetin 7-O-glucoside
-
488817
Citrus x paradisi
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Citrus x paradisi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Citrus x paradisi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
10 mM, strong
Citrus x paradisi
ATP
10 mM, strong
Citrus x paradisi
NADPH
10 mM, weak
Citrus x paradisi
TTP
10 mM, strong
Citrus x paradisi
UDP
10 mM, weak
Citrus x paradisi
UTP
10 mM, strong
Citrus x paradisi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.08
-
naringenin
-
Citrus x paradisi
0.11
-
hesperetin
-
Citrus x paradisi
0.13
-
2',4',6',4-tetrahydroxy chalcone
-
Citrus x paradisi
0.37
-
UDPglucose
-
Citrus x paradisi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
CaCl2, MnCl2, NaCl, MgCl2 at 0.1 mM, 1.0 mM and 10 mM have no effect
Citrus x paradisi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
trivial name naringenin
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
-
Citrus x paradisi
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Citrus x paradisi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
highest activity in leaves, more than in stem and roots
Citrus x paradisi
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 1% bovine serum albumin, 2-3 days, no loss of activity
Citrus x paradisi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + hesperetin
-
488817
Citrus x paradisi
UDP + hesperetin 7-O-glucoside
-
488817
Citrus x paradisi
?
UDP-glucose + naringenin
-
488817
Citrus x paradisi
UDP + naringenin 7-O-glucoside
-
-
-
-
UDPglucose + 2',4',6',4-tetrahydroxychalcone
64% of activity with naringenin
488817
Citrus x paradisi
UDP + 2',4',6',4-tetrahydroxychalcone 4'-O-glucoside
-
488817
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
trivial name naringenin
488817
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
-
488817
Citrus x paradisi
-
UDPglucose + kaempferol
90% of activity with naringenin
488817
Citrus x paradisi
UDP + kaempferol 7-O-glucoside
-
488817
Citrus x paradisi
?
UDPglucose + quercetin
164% of activity with naringenin
488817
Citrus x paradisi
UDP + quercetin 7-O-glucoside
-
488817
Citrus x paradisi
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Citrus x paradisi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
-
Citrus x paradisi
Other publictions for EC 2.4.1.185
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705189
Noguchi
-
cDNA cloning of glycosyltransf ...
Lycium barbarum
J. Mol. Catal. B
55
84-92
2008
-
-
-
-
-
-
3
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
1
1
488819
Durren
Flavanone-7-O-glucosyltransfer ...
Petunia x hybrida
Phytochemistry
52
793-798
1999
-
-
-
-
-
-
1
5
-
1
1
1
-
1
-
-
1
-
-
3
-
-
7
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
5
-
1
1
1
-
-
-
1
-
3
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488816
McIntosh
Flavanone-specific 7-O-glucosy ...
Citrus x paradisi
Arch. Biochem. Biophys.
282
50-57
1990
-
-
-
-
-
-
-
3
-
-
1
2
-
1
-
-
1
-
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
2
-
-
-
1
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
488817
McIntosh
-
Biosynthesis of naringin in Ci ...
Citrus x paradisi
Phytochemistry
29
1533-1538
1990
-
-
-
-
-
-
6
4
-
1
-
1
-
1
-
-
1
-
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
-
1
-
1
-
-
-
1
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488818
Lewinsohn
Flavanone glycoside biosynthes ...
Citrus maxima, Citrus mitis
Plant Physiol.
91
1323-1328
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
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-
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