BRENDA - Enzyme Database
show all sequences of 2.4.1.185

Flavanone-specific 7-O-glucosyltransferase activity in Citrus paradisi seedlings: purification and characterization

McIntosh, C.A.; Latchinian, L.; Mansell, R.L.; Arch. Biochem. Biophys. 282, 50-57 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.063
-
naringenin
-
Citrus x paradisi
0.124
-
hesperetin
-
Citrus x paradisi
0.243
-
UDPglucose
-
Citrus x paradisi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54900
-
gel filtration
Citrus x paradisi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
enzyme does not accept other flavanone or flavonol aglycones
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
trivial name naringenin
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
Citrus x paradisi
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Citrus x paradisi
-
grapefruit
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, Sephadex G-100, hydroxyapatite, UDP-glucuronic acid agarose, Mono Q, Mono P
Citrus x paradisi
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Citrus x paradisi
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0017
-
-
Citrus x paradisi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + hesperetin
trivial name hesperetin, 83% of activity with naringenin
488816
Citrus x paradisi
UDP + hesperetin 7-O-glucoside
-
488816
Citrus x paradisi
?
UDP-glucose + naringenin
-
488816
Citrus x paradisi
UDP + naringenin 7-O-glucoside
-
-
-
-
UDPglucose + 4',5',7-trihydroxyflavanone
enzyme does not accept other flavanone or flavonol aglycones
488816
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
488816
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
trivial name naringenin
488816
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
488816
Citrus x paradisi
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Citrus x paradisi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
hesperetin
Citrus x paradisi
7.5
-
naringenin
Citrus x paradisi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
60% of maximal activity at pH 5.5 and pH 7.5
Citrus x paradisi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.063
-
naringenin
-
Citrus x paradisi
0.124
-
hesperetin
-
Citrus x paradisi
0.243
-
UDPglucose
-
Citrus x paradisi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54900
-
gel filtration
Citrus x paradisi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
enzyme does not accept other flavanone or flavonol aglycones
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
Citrus x paradisi
trivial name naringenin
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
Citrus x paradisi
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, Sephadex G-100, hydroxyapatite, UDP-glucuronic acid agarose, Mono Q, Mono P
Citrus x paradisi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Citrus x paradisi
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0017
-
-
Citrus x paradisi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + hesperetin
trivial name hesperetin, 83% of activity with naringenin
488816
Citrus x paradisi
UDP + hesperetin 7-O-glucoside
-
488816
Citrus x paradisi
?
UDP-glucose + naringenin
-
488816
Citrus x paradisi
UDP + naringenin 7-O-glucoside
-
-
-
-
UDPglucose + 4',5',7-trihydroxyflavanone
enzyme does not accept other flavanone or flavonol aglycones
488816
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
488816
Citrus x paradisi
?
UDPglucose + 4',5',7-trihydroxyflavanone
trivial name naringenin
488816
Citrus x paradisi
UDP + 4',5',7-trihydroxyflavanone 7-O-glucoside
trivial name prunin
488816
Citrus x paradisi
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Citrus x paradisi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
hesperetin
Citrus x paradisi
7.5
-
naringenin
Citrus x paradisi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.5
60% of maximal activity at pH 5.5 and pH 7.5
Citrus x paradisi
Other publictions for EC 2.4.1.185
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705189
Noguchi
-
cDNA cloning of glycosyltransf ...
Lycium barbarum
J. Mol. Catal. B
55
84-92
2008
-
-
-
-
-
-
3
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
1
1
1
1
-
-
-
-
-
1
1
488819
Durren
Flavanone-7-O-glucosyltransfer ...
Petunia x hybrida
Phytochemistry
52
793-798
1999
-
-
-
-
-
-
1
5
-
1
1
1
-
1
-
-
1
-
-
3
-
-
7
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
2
5
-
1
1
1
-
-
-
1
-
3
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
488816
McIntosh
Flavanone-specific 7-O-glucosy ...
Citrus x paradisi
Arch. Biochem. Biophys.
282
50-57
1990
-
-
-
-
-
-
-
3
-
-
1
2
-
1
-
-
1
-
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
2
-
-
-
1
-
1
1
-
4
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
488817
McIntosh
-
Biosynthesis of naringin in Ci ...
Citrus x paradisi
Phytochemistry
29
1533-1538
1990
-
-
-
-
-
-
6
4
-
1
-
1
-
1
-
-
1
-
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
-
1
-
1
-
-
-
1
-
1
-
1
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
488818
Lewinsohn
Flavanone glycoside biosynthes ...
Citrus maxima, Citrus mitis
Plant Physiol.
91
1323-1328
1989
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-