Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Parageobacillus thermoglucosidasius |
General Stability | Organism |
---|---|
truncation of the C-terminal extension results in greater stability and solubility than the wild type, as well as a lower sensitivity to the presence of added metal ions | Parageobacillus thermoglucosidasius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | the wild type enzyme shows bout 75% residual activity at 10 mM. The C-terminally truncated enzyme shows about 95% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Ca2+ | the wild type enzyme shows about 90% residual activity at 10 mM. The C-terminally truncated enzyme shows about 97% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Co2+ | the wild type enzyme shows complete inhibition at 3 mM. The C-terminally truncated enzyme shows about 28% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Cr2+ | the wild type enzyme shows about 75% residual activity at 10 mM. The C-terminally truncated enzyme shows about 94% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Cu2+ | the wild type enzyme shows about 10% residual activity at 10 mM. The C-terminally truncated enzyme shows about 51% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Fe2+ | the wild type enzyme shows about 85% residual activity at 10 mM. The C-terminally truncated enzyme shows about 97% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Fe3+ | the wild type enzyme shows about 78% residual activity at 10 mM. The C-terminally truncated enzyme shows about 95% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Mn2+ | the wild type enzyme shows about 35% residual activity at 10 mM. The C-terminally truncated enzyme shows about 69% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Ni2+ | the wild type enzyme shows about 2% residual activity at 10 mM. The C-terminally truncated enzyme shows about 25% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Zn2+ | the wild type enzyme shows about 18% residual activity at 10 mM. The C-terminally truncated enzyme shows about 58% residual activity at 10 mM | Parageobacillus thermoglucosidasius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | the wild type enzyme shows 100% activity at 1 mM. The C-terminally truncated enzyme shows about 102% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Ca2+ | the C-terminally truncated enzyme shows about 105% activity at 1 mM | Parageobacillus thermoglucosidasius | |
K+ | the wild type enzyme shows about 128% activity at 1 mM. The C-terminally truncated enzyme shows about 103% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Mg2+ | the wild type and C-terminally truncated enzyme show 100% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Na+ | the wild type enzyme shows about 118% activity at 1 mM. The C-terminally truncated enzyme shows about 106% activity at 1 mM | Parageobacillus thermoglucosidasius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | A0A068F9H3 | - |
- |
Parageobacillus thermoglucosidasius STB02 | A0A068F9H3 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap ANX column chromatography and HiTrap phenyl column chromatography | Parageobacillus thermoglucosidasius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin | - |
Parageobacillus thermoglucosidasius | ? | - |
? | |
amylopectin | - |
Parageobacillus thermoglucosidasius STB02 | ? | - |
? | |
amylose | - |
Parageobacillus thermoglucosidasius | ? | - |
? | |
amylose | - |
Parageobacillus thermoglucosidasius STB02 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GBE | - |
Parageobacillus thermoglucosidasius |