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Literature summary for 2.4.1.18 extracted from

  • Yoon, S.A.; Ryu, S.I.; Lee, S.B.; Moon, T.W.
    Purification and characterization of branching specificity of a novel extracellular amylolytic enzyme from marine hyperthermophilic Rhodothermus marinus (2008), J. Microbiol. Biotechnol., 18, 457-464.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl concentration of 0.5 - 1 M NaCl Rhodothermus marinus

Inhibitors

Inhibitors Comment Organism Structure
DMSO about 22.9% decreased enzyme activity, 10% Rhodothermus marinus
EDTA about 44.2% decreased enzyme activity, 5 mM Rhodothermus marinus
ethanol about 57.2% decreased enzyme activity, 10% Rhodothermus marinus
methanol about 45.2% decreased enzyme activity, 10% Rhodothermus marinus
NaCl concetration of 2-4 M NaCl Rhodothermus marinus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Rhodothermus marinus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 2.5% decreased enzyme activity, 5 mM Rhodothermus marinus
Ca2+ about 3.6% decreased enzyme activity, 5 mM Rhodothermus marinus
Cu2+ about 16.4% decreased enzyme activity, 5 mM Rhodothermus marinus
Fe2+ about 58.3% decreased enzyme activity, 5 mM Rhodothermus marinus
Li+
-
Rhodothermus marinus
Mg2+ 15% increased enzyme activity, 5 mM Rhodothermus marinus
Mn2+ about 53.5% decreased enzyme activity, 5 mM Rhodothermus marinus
Zn2+ about 61.8% decreased enzyme activity, 5 mM Rhodothermus marinus

Organism

Organism UniProt Comment Textmining
Rhodothermus marinus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Q-Sepharose column, ultrafiltration, gel filtration with Superdex 200 column, native polyacrylamide gel elektrophoresis Rhodothermus marinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose dinitrosalicylate method, assay at pH 6.0, 70°C Rhodothermus marinus ?
-
?
Maltohexaose dinitrosalicylate method, assay at pH 6.0, 70°C Rhodothermus marinus ?
-
?
maltopentaose dinitrosalicylate method, assay at pH 6.0, 70°C Rhodothermus marinus ?
-
?
Pullulan dinitrosalicylate method, assay at pH 6.0, 70°C Rhodothermus marinus ?
-
?

Synonyms

Synonyms Comment Organism
RMEBE
-
Rhodothermus marinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Rhodothermus marinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90
-
Rhodothermus marinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min Rhodothermus marinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Rhodothermus marinus

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
Rhodothermus marinus