Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DG5alpha | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the cation has no effect on the activity. The reaction mixture is incubated in the presence of 10 mM of the cation for 30 min | Mycobacterium tuberculosis | |
Cu2+ | maximum inhibition is observed, where about 40% of the activity is retained. The reaction mixture is incubated in the presence of 10 mM of the cation for 30 min | Mycobacterium tuberculosis | |
Mg2+ | the cation has no effect on the activity. The reaction mixture is incubated in the presence of 10 mM of the cation for 30 min | Mycobacterium tuberculosis | |
Zn2+ | about 70% activity retained. The reaction mixture is incubated in the presence of 10 mM of the cation for 30 min | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration chromatography using Superdex 200 HR10/30 column | Mycobacterium tuberculosis |
85000 | - |
SDS-PAGE at 4°C | Mycobacterium tuberculosis |
86060 | - |
theoretical mass of the recombinant branching enzyme with N-terminal S-tag and C-terminal His-tag | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WN45 | the primary amino acid sequence has four cysteine residues at position: 95, 193, 617 and 658, therefore there is a possibility of forming intramoleculare disulfide bonds. The two closely migrating bands of purified protein on a non-reducing SDS-PAGE might be due to the presence of two populations of different confomations of the same protein: the oxidized and the reduced.; H37Rv | - |
Mycobacterium tuberculosis H37Rv | P9WN45 | the primary amino acid sequence has four cysteine residues at position: 95, 193, 617 and 658, therefore there is a possibility of forming intramoleculare disulfide bonds. The two closely migrating bands of purified protein on a non-reducing SDS-PAGE might be due to the presence of two populations of different confomations of the same protein: the oxidized and the reduced.; H37Rv | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity column and Q-Sepharose column | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GlgB | glycogen branching enzyme | Mycobacterium tuberculosis |
ORF Rv1326c | in Mycobacterium tuberculosis H37Rv codes for a enzymatically active protein that utilizes amylose as the substrate | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
incubated at different temperatures for 30 min at pH 7 in 50 mM citrate buffer | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | pre-incubation of the enzyme for 2 h at different temperatures, following enzyme assay is conducted at 30°C. About 80% activity is retained after pre-incubation at 37°C and 45°C | Mycobacterium tuberculosis |
45 | - |
with a direct enzyme assay at 45°C the enzyme retains about 45% activity | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
all assays are perfomed at 30°C with 0.5 microM of protein | Mycobacterium tuberculosis |