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Literature summary for 2.4.1.18 extracted from
- Biosynthesis of bacterial glycogen. Purification and properties of the Escherichia coli b alpha-1,4-glucan: alpha-1,4-glucan 6-glycosyltansferase (1977), Biochemistry, 16, 3693-3699.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
1 * 84000, SDS-PAGE | Escherichia coli |
| 87000 | - |
branching enzyme II, sucrose density gradient centrifugation | Escherichia coli |
| 95000 | - |
branching enzyme I, sucrose density gradient centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 299 | - |
branching enzyme II | Escherichia coli |
| 460 | - |
branching enzyme I | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C or 4°C, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin | - |
Escherichia coli | amylopectin with additional alpha-1,6-glucosidic linkages | - |
? |  |
| amylose | - |
Escherichia coli | amylose containing alpha-1,6-glucosidic linkages | characterization of products, smallest chains transferred contain 5-7 glucose units | ? | |
| Glycogen | from E. coli and rabbit liver, little activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 84000, SDS-PAGE | Escherichia coli |
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