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Literature summary for 2.4.1.179 extracted from
- Characterization and membrane organization of beta 1-3- and beta 1-4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures (1989), Arch. Biochem. Biophys., 270, 630-646.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Bovine liver non-specific transfer proteins | activation | Homo sapiens | |
| CHAPSO | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens |  |
| deoxycholate | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens | |
| additional information | little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A | Homo sapiens | |
| phosphatidylethanolamine | activation, less efficient than Triton CF-54 | Homo sapiens | |
| taurodeoxycholate | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens | |
| Triton CF-54 | activation, optimal at 0.1% | Homo sapiens | |
| Triton X-100 | activation, can replace Triton CF-54 with 75% less efficiency | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
lactotriaosylceramide | - |
Homo sapiens | |
| 0.048 | - |
UDP-galactose | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, can replace Mn2+ with 26% efficiency | Homo sapiens | |
| Co2+ | activation, can replace Mn2+ with 51% efficiency | Homo sapiens | |
| Cu2+ | activation, can replace Mn2+ with 15% efficiency | Homo sapiens | |
| Mg2+ | activation, can replace Mn2+ with 13% efficiency | Homo sapiens | |
| Mn2+ | requirement, 25 mM | Homo sapiens | |
| additional information | no activation by Zn2+ or Cd2+ | Homo sapiens | |
| Ni2+ | activation, can replace Mn2+ with 16% efficiency | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid | Homo sapiens | associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from Colo 205 cell line | Homo sapiens |
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Homo sapiens | - |
| Colo-205 cell | colonic adenocarcino cell line | Homo sapiens | - |
| colonic adenocarcinoma cell | - |
Homo sapiens | - |
| SW-403 cell | colonic adenocarcino cell line | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00077 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid | associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure | Homo sapiens | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid | - |
? | |
| UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R | substrate specificity | Homo sapiens | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R | - |
? | |
| UDP-galactose + lactotriaosylceramide | best substrate | Homo sapiens | UDP + lactotetraosylceramide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
Hepes buffer | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.2 | about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer | Homo sapiens |
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