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Literature summary for 2.4.1.177 extracted from
- Cinnamate metabolism in ripening fruit. Characterization of a UDP-glucose:cinnamate glucosyltransferase from strawberry (2006), Plant Physiol., 140, 1047-1058.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) pLysS | Fragaria x ananassa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transgenic strawberry plants expressing the FaGT2 gene in antisense orientation under the control of the cauliflower mosaic virus 35S promotor. Two of the transgenic strawberry lines contain reduced levels of cinnamoyl- and p-coumaroyl-D-glucose in comparison with the control group. The mutants do not significantly differ in the concentration of caffeoyl-, feruloyl-, and p-hydroxylbenzoyl-D-glucose, when compared with controls. | Fragaria x ananassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0808 | - |
UDP-glucose | with cinnamic acid | Fragaria x ananassa |  |
| 0.108 | - |
3,4-dimethoxycinnamic acid | - |
Fragaria x ananassa | |
| 0.3002 | - |
Sinapic acid | - |
Fragaria x ananassa | |
| 0.3157 | - |
5-hydroxyferulic acid | - |
Fragaria x ananassa | |
| 0.3569 | - |
Cinnamic acid | - |
Fragaria x ananassa | |
| 0.3585 | - |
ferulic acid | - |
Fragaria x ananassa | |
| 0.4112 | - |
phenylpropionic acid | - |
Fragaria x ananassa | |
| 0.431 | - |
phenylbutyric acid | - |
Fragaria x ananassa | |
| 0.4371 | - |
4-Aminobenzoic acid | - |
Fragaria x ananassa | |
| 0.4644 | - |
3-Hydroxybenzoic acid | - |
Fragaria x ananassa | |
| 0.4882 | - |
3-aminobenzoic acid | - |
Fragaria x ananassa | |
| 0.5026 | - |
benzoic acid | - |
Fragaria x ananassa | |
| 0.5153 | - |
vanillic acid | - |
Fragaria x ananassa | |
| 0.6035 | - |
p-coumaric acid | - |
Fragaria x ananassa | |
| 0.6424 | - |
p-hydroxybenzoic acid | - |
Fragaria x ananassa | |
| 0.7077 | - |
caffeic acid | - |
Fragaria x ananassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the addition of 1 mM leads to a loss of 30% to 50%, the addition to 10 mM causes a loss of more than 90% of activity | Fragaria x ananassa | |
| Fe2+ | the addition of 1 mM leads to a loss of 30% to 50%, the addition to 10 mM causes a loss of more than 60% of activity | Fragaria x ananassa | |
| Mg2+ | has no effect at 10 mM | Fragaria x ananassa | |
| Mn2+ | the addition to 10 mM causes a loss of 30% of activity | Fragaria x ananassa | |
| Zn2+ | the addition of 1 mM leads to a loss of 30% to 50%, the addition to 10 mM causes a loss of more than 90% of activity | Fragaria x ananassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61400 | - |
estimated molecular mass | Fragaria x ananassa |
| 65000 | - |
FaGT2 fusion protein, SDS-PAGE | Fragaria x ananassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fragaria x ananassa | Q66PF4 | FaGT2 gene; cv Calypso or Elsanta, strawberry. Gene encoding the enzyme in strawberry. The expression of the FaGT2 gene is negatively regulated by auxin. Expression of the FaGT2 gene is induced by oxidative stress treatment in both strawberry fruit and strawberry cell cultures. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fragaria x ananassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | FaGT2 mRNA is detectable in flowers | Fragaria x ananassa | - |
| fruit | at different ripening stages, the amount of FaGT2 mRNA increases strongly along the fruit-ripening stages, with maximal expression value in fully ripe red fruit. | Fragaria x ananassa | - |
| leaf | FaGT2 mRNA is barely detectable in leaves | Fragaria x ananassa | - |
| petiole | FaGT2 mRNA is detectable in petioles | Fragaria x ananassa | - |
| root | FaGT2 mRNA is barely detectable in roots | Fragaria x ananassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax is 2.34 nkat/mg for cinnamic acid, 2.69 nkat/mg for p-coumaric acid, 2.59 nkat/mg for caffeic acie, 1.65 nkat/mg for ferulic acid, 2.24 nkat/mg for 5-hydroxyferulic acid, 1.21 nkat/mg for sinapic acid, 1.08 nkat/mg for benzoic acid, 1.05 nkat/mg for 3-hydroxybenzoic acid, 0.77 nkat/mg for p-hydroxybenzoic acid, 1.69 nkat/mg for vanillic acid, 0.68 nkat/mg for 3,4-dimethoxycinnamic acid, 0.17 nkat/mg for phenylpropionic acid, 0.22 nkat/mg for phenylbutyric acid, 0.75 nkat/mg for 3-aminobenzoic acid, 1.73 nkat/mg for 4-aminobenzoic acid and 4.88 nkat/mg for UDP-glucose (with cinnamic acid). Enzyme activity is assayed in 100 mM Tris-HCL buffer, pH 8.0, containing 5 mM 2-mercaptoethanol, 10% glycerol, and 5 mM sodium disulfite. The aglycon concentration is varied from 2 microM to 2 mM, at constant (5 mM) UDP-Glc concentration at 21°C. | Fragaria x ananassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dimethoxycinnamic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 3,4-dimethoxycinnamoyl-beta-D-glucoside | - |
? | |
| 3-aminobenzoic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 3-aminobenzoyl-beta-D-glucoside | - |
? | |
| 3-hydroxybenzoic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 1-O-(3-hydroxybenzoyl)-beta-D-glucopyranose | - |
? | |
| 4-aminobenzoic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 4-aminobenzoyl-beta-D-glucoside | - |
? | |
| 5-hydroxyferulic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 5-hydroxyferuloyl-beta-D-glucoside | - |
? | |
| benzoic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + benzoyl-beta-D-glucoside | - |
? | |
| caffeic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + caffeoyl-beta-D-glucoside | - |
? | |
| cinnamic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + cinnamoyl-beta-D-glucoside | - |
? | |
| ferulic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + feruloyl-beta-D-glucoside | - |
? | |
| additional information | only compounds containing a carboxylic group and an aromatic ring structure are accepted as substrates. Substituents at the aromatic ring are tolerated as long as they are not located in the ortho position. | Fragaria x ananassa | ? | - |
? | |
| p-coumaric acid + UDP-glucose | - |
Fragaria x ananassa | UDP + p-coumaroyl-beta-D-glucoside | - |
? | |
| p-hydroxybenzoic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + 4-hydroxybenzoyl-beta-D-glucoside | - |
? | |
| phenylbutyric acid + UDP-glucose | - |
Fragaria x ananassa | UDP + phenylbutyryl-beta-D-glucoside | - |
? | |
| phenylpropionic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + phenylpropionyl-beta-D-glucoside | - |
? | |
| sinapic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + sinapoyl-beta-D-glucoside | - |
? | |
| vanillic acid + UDP-glucose | - |
Fragaria x ananassa | UDP + vannilyl-beta-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FaGT2 | - |
Fragaria x ananassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
- |
Fragaria x ananassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Fragaria x ananassa |
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Release 2023.1 (January 2023)
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