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Literature summary for 2.4.1.174 extracted from

  • Saigoh, K.; Yoshimura, S.; Izumikawa, T.; Miyata, S.; Tabara, Y.; Matsushita, T.; Miki, T.; Miyamoto, K.; Hirano, M.; Kitagawa, H.; Kira, J.I.; Kusunoki, S.
    Chondroitin sulfate beta-1,4-N-acetylgalactosaminyltransferase-1 (ChGn-1) polymorphism: association with progression of multiple sclerosis (2016), Neurosci. Res., 108, 55-59.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CSGALNACT1, genotyping and polymorphisms, recombinant expression of His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant in COS-1 cells, the enzymes are secreted Homo sapiens

Protein Variants

Protein Variants Comment Organism
S126L naturally occuring inactive ChGN1 mutant Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, about, recombinant His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant enzymes, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan Homo sapiens
-
UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TDX6 gene csgalnact1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant from COS-1 cellsby nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan
-
Homo sapiens UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan
-
?

Subunits

Subunits Comment Organism
? x * 70000, about, recombinant His-tagged soluble forms of wild-type ChGn-1 and S126LChGn-1 mutant enzymes, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
chondroitin sulfate beta-1,4-N-acetylgalactosaminyltransferase-1 ChGn-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function ChGn-1 is a key enzyme for production of chondroitin sulfate proteoglycans The clinical course of multiple sclerosis is influenced by the level of expression of the ChGn-1 gene. Chondroitin sulfate proteoglycans are a constituent of the matrix of the central nervous system (CNS), likely participating as regulatory molecules in the process of demyelination, remyelination, axonaldegeneration and regeneration in the CNS Homo sapiens