Literature summary for 2.4.1.174 extracted from

Sakai, K.; Kimata, K.; Sato, T.; Gotoh, M.; Narimatsu, H.; Shinomiya, K.; Watanabe, H.
Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage (2007), J. Biol. Chem., 282, 4152-4161.

Search for more literature for 2.4.1.174

Cloned(Commentary)

Cloned (Comment)	Organism
LTC cells from rat chondrosarcoma are transfected with chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression plasmid (about 80fold increase of chondroitin sulfate N-acetylgalactosaminyltransferase-1 RNA), metabolic albeling with [35S]sulfate show up to a 2.2fold increase in chondroitin sulfate levels in these cells, but the mRNA level of aggrecan core protein does not change. Aggrecan obained from chondroitin sulfate N-acetylgalactosaminyltransferase-1 overexpressing cells contain a larger amount of chondroitin sulfate, but the chondroitin sulfate chain length is similar to mock-transfected cells.	Homo sapiens

Cloned (Comment)

Organism

LTC cells from rat chondrosarcoma are transfected with chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression plasmid (about 80fold increase of chondroitin sulfate N-acetylgalactosaminyltransferase-1 RNA), metabolic albeling with [35S]sulfate show up to a 2.2fold increase in chondroitin sulfate levels in these cells, but the mRNA level of aggrecan core protein does not change. Aggrecan obained from chondroitin sulfate N-acetylgalactosaminyltransferase-1 overexpressing cells contain a larger amount of chondroitin sulfate, but the chondroitin sulfate chain length is similar to mock-transfected cells.

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	in cmd mice (known as natural aggrecan-null mice) the heterozygote and homozygote cartilage exhibit about 50% and about 9% aggrecan gene transcription respectively. In the cmd heterozygote cartilage, chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression is dimnished to about 30% that of the wild type. In the cmd homozygote cartilage, chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression furhter decreases to a low level.	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	transfected cells are injected into the cuadal intervertebral discs of 4-month old ICR mice, analyses at day seven after the injection show, that in vivo gene delivery of chondroitin sulfate N-acetylgalactosaminyltransferase-1 increases chondroitin sulfate biosynthesis in cartilage.	-
Mus musculus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine	the enzyme mainly regulates CS synthesis in cartilage	Mus musculus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
bone	-	Homo sapiens	-
additional information	the enzyme is expressed (at very high levels) in the prehypertrophic zone of the growth plate at E16.5, colocalized with aggrecan core protein. In ATDC5 cells the expression of CSGlcAT and CSGalNAcT-1 correlate well with that of aggrecan core protein. Real time RT-PCR show a high level of chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression in cartilage at E18.5.	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
CSGalNAcT-1	-	Mus musculus
CSGalNAcT-1	chondroitin sulfate N-acetylgalactosaminyltransferase-1	Homo sapiens