Literature summary for 2.4.1.174 extracted from

Uyama, T.; Kitagawa, H.; Tanaka, J.; Tamura, J.i.; Ogawa, T.; Sugahara, K.
Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate (2003), J. Biol. Chem., 278, 3072-3078.

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Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	type II membrane protein topology	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	x * 95000, recombinant enzyme, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8N6G5	-	-
Homo sapiens	Q8TDX6	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	2 potential N-glycosylation sites, either one or both of which are used in COS-1 cells during expression of the recombinant fusion protein	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
leukocyte	peripheral blood	Homo sapiens	-
lung	-	Homo sapiens	-
peripheral blood	-	Homo sapiens	-
skeletal muscle	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)	-	Homo sapiens	UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)	-	?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser	-	Homo sapiens	UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser	-	?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide	-	Homo sapiens	UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide	-	?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl	-	Homo sapiens	UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl	-	?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides	-	Homo sapiens	UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides	-	?

Subunits

Subunits	Comment	Organism
?	x * 95000, recombinant enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
chondroitin galactosaminyltransferase	-	Homo sapiens
More	-	Homo sapiens
More	the enzyme has also N-acetylgalactosaminyltransferase II activity, EC 2.4.1.175, substrates sulfated and unsulfated chondroitin oligo- and polysaccharides	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)