Cloned (Comment) | Organism |
---|---|
gene UGT80B1, recombinant expression of native promoter and 35 S driven GFP-tagged wild-type and mutant enzymes, the ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of ugt80B1 mutants. Sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only. Root hair patterning and GL2 expression are aberrant in ugt80B1 mutants. Expression of upstream cell fate regulators, SCM and WER, is altered in ugt80B1 mutants. Phenotype, overview. The ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
hechtian strand | - |
Arabidopsis thaliana | - |
- |
additional information | UGT80B1:GFP is localized in the hechtian strands, connecting the retracting protoplast from the cell wall. Wild-type exhibits clear cell periphery localization of SCM:GFP in one of the two cell files, differentiating developing H cells from N cells. In ugt80B1 mutants, a decrease in cell peripheral localization of SCM:GFP can be seen, and signal is detected in both H and N cell files indicating a loss in cell-type specific accumulation of SCRAMBLED (SCM). An increase in cytoplasmic punctae labeled with SCM:GFP is visible in both cell files in ugt80B1 | Arabidopsis thaliana | - |
- |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a sterol | Arabidopsis thaliana | - |
UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + a sterol | Arabidopsis thaliana Col-0 | - |
UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | Arabidopsis thaliana | - |
UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | Arabidopsis thaliana Col-0 | - |
UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9XIG1 | - |
- |
Arabidopsis thaliana Col-0 | Q9XIG1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Arabidopsis thaliana | - |
hairy root | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
seed | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a sterol | - |
Arabidopsis thaliana | UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + a sterol | - |
Arabidopsis thaliana Col-0 | UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | - |
Arabidopsis thaliana | UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | - |
Arabidopsis thaliana Col-0 | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, recombinant GFP-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
UDP-Glc:sterol glucosyltransferase 80B1 | - |
Arabidopsis thaliana |
UGT80B1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the genome of Arabidopsis thaliana contains two genes coding for UDP-Glc:sterol-glucosyltransferases, UGT80A2 and UGT80B1, and studies of mutant lines indicate that they are only partially redundant | Arabidopsis thaliana |
malfunction | the plasma membrane cell fate regulator, SCRAMBLED (SCM), is mislocalized in ugt80B1 mutants, underscoring the aberrant root epidermal cell patterning. GFP-tagged SCM is localized to the cytoplasm in a non cell type dependent manner instead of the hair (H) cell plasma membrane in these mutants. Abnormal root hair cell patterning in ugt80B1 mutants is likely the direct result of expression of GL2 in H cell files in this mutant. The aberrant expression of GL2 is caused by the mislocalization of SCM away from the cell periphery, reducing the capacity of the receptor to mediate positional information to the cell. Reductions in specific sterol glucosides might be responsible for the disruption of cell fate regulators in these ugt80B1 mutants. The mislocalization of SCM to the cytoplasm can point to a role for sterol glucosides in vesicular trafficking or plasma membrane protein targeting. Deficiencies in specific sterol glucosides are sufficient to disrupt normal cell function and point to a possible role for sterol glucosides in cargo transport and/or protein targeting to the plasma membrane. Aberrant subcellular localization of SCM:GFP in ugt80B1 epidermal cells from the elongation zone of the root | Arabidopsis thaliana |
additional information | sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only | Arabidopsis thaliana |