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Literature summary for 2.4.1.173 extracted from

  • Castillo, N.; Pastor, V.; Chavez, A.; Arro, M.; Boronat, A.; Flors, V.; Ferrer, A.; Altabella, T.
    Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea (2019), Front. Plant Sci., 10, 1162 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene UGT80A2, quantitative reverse-transcription PCR expression analysis Arabidopsis thaliana
gene UGT80B1, quantitative reverse-transcription PCR expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview Arabidopsis thaliana
additional information generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + a sterol Arabidopsis thaliana
-
UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + a sterol Arabidopsis thaliana Ws-0
-
UDP + a sterol 3-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M8Z7 Wassilewskija (Ws-0) ecotype
-
Arabidopsis thaliana Q9XIG1 Wassilewskija (Ws-0) ecotype
-
Arabidopsis thaliana Ws-0 Q9M8Z7 Wassilewskija (Ws-0) ecotype
-
Arabidopsis thaliana Ws-0 Q9XIG1 Wassilewskija (Ws-0) ecotype
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + a sterol
-
Arabidopsis thaliana UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + a sterol
-
Arabidopsis thaliana Ws-0 UDP + a sterol 3-beta-D-glucoside
-
?

Synonyms

Synonyms Comment Organism
UDP-glucose sterol glucosyltransferase
-
Arabidopsis thaliana
UGT80A2
-
Arabidopsis thaliana
UGT80B1
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis thaliana resistance to Botrytis cinerea infection, which correlates with increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Analysis of the response to necrotrophic fungus Botrytis cinerea in an Arabidopsis thaliana mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases, UGT80A2 and UGT80B1. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also upregulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants Arabidopsis thaliana
metabolism crosstalk between hormone signaling pathways, particularly those mediated by salicylate and jasmonate, has been found to contribute to plant resistance to different types of pathogens. The results suggest that the salicylate-mediated defense pathway is not involved in the response of the ugt80A2;B1 mutant to Bortrytis cinerea infection. But camalexin and, probably, also indole glucosinolates are actively involved in the enhanced resistance of the ugt80A2;B1 mutant to Bortrytis cinerea infection. The synthesis of alkylglucosinolates in the ugt80A2;B1 mutant is not affected by Bortrytis cinerea infection Arabidopsis thaliana