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Literature summary for 2.4.1.173 extracted from

  • Li, X.; Xia, T.; Huang, J.; Guo, K.; Liu, X.; Chen, T.; Xu, W.; Wang, X.; Feng, S.; Peng, L.
    Distinct biochemical activities and heat shock responses of two UDP-glucose sterol glucosyltransferases in cotton (2014), Plant Sci., 219-220, 1-8.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates the isozyme Gossypium hirsutum
Triton activates isozyme GhSGT2 Gossypium hirsutum

Cloned(Commentary)

Cloned (Comment) Organism
gene GhSGT1, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2 Gossypium hirsutum
gene GhSGT2, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2 Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slight inhibition of the isozyme Gossypium hirsutum
Triton strong inhibition of isozyme GhSGT1 Gossypium hirsutum
Zn2+
-
Gossypium hirsutum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Gossypium hirsutum 5737
-
additional information the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2 Gossypium hirsutum
-
-
plasma membrane the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2 Gossypium hirsutum 5886
-
protoplast
-
Gossypium hirsutum
-
-
soluble
-
Gossypium hirsutum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates up to twofold Gossypium hirsutum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + sitosterol Gossypium hirsutum
-
UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol Gossypium hirsutum Huamian99
-
UDP + sitosterol 3-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum A0A023NFQ4 gene GhSGT2
-
Gossypium hirsutum A0A023NGA8 gene GhSGT1
-
Gossypium hirsutum Huamian99 A0A023NFQ4 gene GhSGT2
-
Gossypium hirsutum Huamian99 A0A023NGA8 gene GhSGT1
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Gossypium hirsutum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + sitosterol
-
Gossypium hirsutum UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol
-
Gossypium hirsutum Huamian99 UDP + sitosterol 3-beta-D-glucoside
-
?

Synonyms

Synonyms Comment Organism
SGT1
-
Gossypium hirsutum
SGT2
-
Gossypium hirsutum
UDP-glucose sterol glucosyltransferase
-
Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27 37 assay at Gossypium hirsutum

General Information

General Information Comment Organism
physiological function steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose Gossypium hirsutum
physiological function steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose. Isozyme GhSGT2 mainly participates in catalyzing glucosylation of membrane sterols Gossypium hirsutum