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Literature summary for 2.4.1.173 extracted from

  • Stucky, D.F.; Arpin, J.C.; Schrick, K.
    Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis (2015), J. Exp. Bot., 66, 189-201.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern Arabidopsis thaliana
gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control Arabidopsis thaliana
gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content Arabidopsis thaliana
additional information construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content Arabidopsis thaliana
additional information homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + a sterol Arabidopsis thaliana
-
UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + a sterol Arabidopsis thaliana Col-0
-
UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol Arabidopsis thaliana
-
UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol Arabidopsis thaliana Col-0
-
UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol Arabidopsis thaliana
-
UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol Arabidopsis thaliana Col-0
-
UDP + ergosterol 3-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4KII1 gene At5g24750
-
Arabidopsis thaliana Q9M8Z7 gene At3g07020
-
Arabidopsis thaliana Q9XIG1 gene At1g43620
-
Arabidopsis thaliana Col-0 F4KII1 gene At5g24750
-
Arabidopsis thaliana Col-0 Q9M8Z7 gene At3g07020
-
Arabidopsis thaliana Col-0 Q9XIG1 gene At1g43620
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + a sterol
-
Arabidopsis thaliana UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + a sterol
-
Arabidopsis thaliana Col-0 UDP + a sterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol
-
Arabidopsis thaliana UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol
-
Arabidopsis thaliana Col-0 UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol
-
Arabidopsis thaliana UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol
-
Arabidopsis thaliana Col-0 UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol
-
Arabidopsis thaliana UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol
-
Arabidopsis thaliana Col-0 UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol
-
Arabidopsis thaliana UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol
-
Arabidopsis thaliana Col-0 UDP + stigmasterol 3-beta-D-glucoside
-
?

Synonyms

Synonyms Comment Organism
UGT713B1
-
Arabidopsis thaliana
UGT80A2
-
Arabidopsis thaliana
UGT80B1
-
Arabidopsis thaliana

General Information

General Information Comment Organism
evolution phylogenetic tree of UGT80- and UGT713-related proteins, overview Arabidopsis thaliana
evolution UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview Arabidopsis thaliana
malfunction ugt80B1 mutants displaya a significant reduction only in campesteryl, brassicasteryl, and cholesteryl glucosides, but not sitosteryl or stigmasteryl glucosides Arabidopsis thaliana
physiological function no role in steryl glucoside synthesis for UGT713B1/At5g24750 Arabidopsis thaliana
physiological function UGT80A2 accounts for most of the sitosteryl and stigmasteryl glucoside production in seeds Arabidopsis thaliana
physiological function UGT80B1 plays a specialized role in steryl glucoside synthesis Arabidopsis thaliana