Cloned (Comment) | Organism |
---|---|
gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern | Arabidopsis thaliana |
gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control | Arabidopsis thaliana |
gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content | Arabidopsis thaliana |
additional information | construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content | Arabidopsis thaliana |
additional information | homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a sterol | Arabidopsis thaliana | - |
UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + a sterol | Arabidopsis thaliana Col-0 | - |
UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | Arabidopsis thaliana | - |
UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | Arabidopsis thaliana Col-0 | - |
UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | Arabidopsis thaliana | - |
UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | Arabidopsis thaliana Col-0 | - |
UDP + ergosterol 3-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4KII1 | gene At5g24750 | - |
Arabidopsis thaliana | Q9M8Z7 | gene At3g07020 | - |
Arabidopsis thaliana | Q9XIG1 | gene At1g43620 | - |
Arabidopsis thaliana Col-0 | F4KII1 | gene At5g24750 | - |
Arabidopsis thaliana Col-0 | Q9M8Z7 | gene At3g07020 | - |
Arabidopsis thaliana Col-0 | Q9XIG1 | gene At1g43620 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a sterol | - |
Arabidopsis thaliana | UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + a sterol | - |
Arabidopsis thaliana Col-0 | UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | - |
Arabidopsis thaliana | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | - |
Arabidopsis thaliana Col-0 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | - |
Arabidopsis thaliana | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | - |
Arabidopsis thaliana Col-0 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | - |
Arabidopsis thaliana | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | - |
Arabidopsis thaliana Col-0 | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | - |
Arabidopsis thaliana | UDP + stigmasterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | - |
Arabidopsis thaliana Col-0 | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UGT713B1 | - |
Arabidopsis thaliana |
UGT80A2 | - |
Arabidopsis thaliana |
UGT80B1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic tree of UGT80- and UGT713-related proteins, overview | Arabidopsis thaliana |
evolution | UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview | Arabidopsis thaliana |
malfunction | ugt80B1 mutants displaya a significant reduction only in campesteryl, brassicasteryl, and cholesteryl glucosides, but not sitosteryl or stigmasteryl glucosides | Arabidopsis thaliana |
physiological function | no role in steryl glucoside synthesis for UGT713B1/At5g24750 | Arabidopsis thaliana |
physiological function | UGT80A2 accounts for most of the sitosteryl and stigmasteryl glucoside production in seeds | Arabidopsis thaliana |
physiological function | UGT80B1 plays a specialized role in steryl glucoside synthesis | Arabidopsis thaliana |