Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lecithin | stimulation by cotton and soybean lecithin but not with egg lecithin, appears to increase Vmax with sitosterol and Km of UDP-glucose | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
maleate buffer | strong inhibition at 50 mM | Gossypium hirsutum | |
phosphate buffer | strong inhibition at 50 mM | Gossypium hirsutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
UDP-glucose | lecithin causes a 2fold increase in Km | Gossypium hirsutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane fraction | Gossypium hirsutum | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | - |
cotton | - |
Purification (Comment) | Organism |
---|---|
partial purification by differential centrifugation and DEAE-cellulose column | Gossypium hirsutum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotton fiber | - |
Gossypium hirsutum | - |
seed | - |
Gossypium hirsutum | - |
Storage Stability | Organism |
---|---|
2C, Tris-HCl 50 mM, pH 8.5, two weeks 75% of activity | Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UDP-glucose is an effective glucose donor but not so ADP-glucose, CDP-glucose, TDP-glucose, GDP-glucose, UDP-galactose or UDP-mannose | Gossypium hirsutum | ? | - |
? | |
UDP-glucose + campesterol | - |
Gossypium hirsutum | UDP + campesterol 3-beta-D-glucoside | 4% of the products | ? | |
UDP-glucose + sitosterol | - |
Gossypium hirsutum | UDP + sitosterol 3-beta-D-glucoside | 75% of the product | ? | |
UDP-glucose + stigmasterol | - |
Gossypium hirsutum | UDP + stigmasterol 3-beta-D-glucoside | 6% of the product | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gossypium hirsutum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
Tris-HCl 50 mM, Triton X-100 0.25% | Gossypium hirsutum |