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Literature summary for 2.4.1.173 extracted from

  • Forsee, W.T.; Laine, R.A.; Elbein, A.D.
    Solubilization of a particulate UDP-Glucose:sterol beta-glucosyltransferase in developing cotton fibers and seeds and characterization of steryl 6-acyl-D-glucosides (1974), Arch. Biochem. Biophys., 161, 248-259.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
lecithin stimulation by cotton and soybean lecithin but not with egg lecithin, appears to increase Vmax with sitosterol and Km of UDP-glucose Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
maleate buffer strong inhibition at 50 mM Gossypium hirsutum
phosphate buffer strong inhibition at 50 mM Gossypium hirsutum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
UDP-glucose lecithin causes a 2fold increase in Km Gossypium hirsutum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane fraction Gossypium hirsutum 16020
-

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum
-
cotton
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and DEAE-cellulose column Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
cotton fiber
-
Gossypium hirsutum
-
seed
-
Gossypium hirsutum
-

Storage Stability

Storage Stability Organism
2C, Tris-HCl 50 mM, pH 8.5, two weeks 75% of activity Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDP-glucose is an effective glucose donor but not so ADP-glucose, CDP-glucose, TDP-glucose, GDP-glucose, UDP-galactose or UDP-mannose Gossypium hirsutum ?
-
?
UDP-glucose + campesterol
-
Gossypium hirsutum UDP + campesterol 3-beta-D-glucoside 4% of the products ?
UDP-glucose + sitosterol
-
Gossypium hirsutum UDP + sitosterol 3-beta-D-glucoside 75% of the product ?
UDP-glucose + stigmasterol
-
Gossypium hirsutum UDP + stigmasterol 3-beta-D-glucoside 6% of the product ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gossypium hirsutum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
Tris-HCl 50 mM, Triton X-100 0.25% Gossypium hirsutum