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Literature summary for 2.4.1.173 extracted from
- Uridine diphosphate glucose-sterol glucosyltransferase and nucleoside diphosphatase activities in etiolated pea seedlings (1978), Biochem. J., 169, 297-303.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 70% of inhibition at 13 mM | Pisum sativum |  |
| EGTA | 50% of inhibition at 13 mM | Pisum sativum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cetyltrimethylammonium bromide | strong inhibition | Pisum sativum | |
| GDP-glucose | 20% of inhibition at high concentration | Pisum sativum | |
| additional information | not inhibited by ADP, GDP or inorganic phosphorus | Pisum sativum | |
| sodium deoxycholate | strong inhibition at 0.2-0.8% | Pisum sativum | |
| Triton X-100 | moderate inhibition but strong inhibition at low concentrations | Pisum sativum | |
| UDP | 60% of inhibition | Pisum sativum | |
| UMP | 20% of inhibition | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | denser class of Golgi apparatus | Pisum sativum | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulated at 2-25 mM to a greater extent than Mg 2+ | Pisum sativum | |
| Mg2+ | stimulated at 2-25 mM | Pisum sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
pea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification by differential centrifugation | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | etiolated | Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UDP-glucose is an effective glucose donor, not so ADP-glucose, CDP-glucose or GDP-glucose | Pisum sativum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pisum sativum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
Tris-HCl 50 mM | Pisum sativum |
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