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Literature summary for 2.4.1.173 extracted from
- Steroid-specific glucosyltransferase in Asparagus plumosus shoots (1990), Phytochemistry, 29, 63-70.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slightly stimulation | Asparagus setaceus |  |
| p-chloromercuribenzoate | potent inhibitory effect indicating that free SH groups are essential for activity | Asparagus setaceus | |
| Triton X-100 | activity stimulated at 0.1-0.5% | Asparagus setaceus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | potent inhibitory effect at 0.1 mM indicating that free SH groups are essential for activity | Asparagus setaceus | |
| UDP | total inhibition at 1 mM | Asparagus setaceus | |
| UMP | inhibition at 1 mM | Asparagus setaceus | |
| UTP | total inhibition at 1 mM | Asparagus setaceus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Asparagus setaceus | 5737 | - |
| membrane | membrane fraction | Asparagus setaceus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibitory effect at 1 mM | Asparagus setaceus | |
| Hg2+ | inhibitory effect at 1 mM | Asparagus setaceus | |
| Mg2+ | not stimulated by 1 mM | Asparagus setaceus | |
| Mn2+ | no effect at 1 mM | Asparagus setaceus | |
| additional information | no metal ion requirement | Asparagus setaceus | |
| Zn2+ | inhibitory effect at 1 mM | Asparagus setaceus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Asparagus setaceus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| shoot | - |
Asparagus setaceus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | yamogenin is not substrate of this enzyme | Asparagus setaceus | ? | - |
? | |
| UDP-glucose + sitosterol | - |
Asparagus setaceus | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Asparagus setaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
Tris-HCl 0.1 M | Asparagus setaceus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.7 | - |
Asparagus setaceus |
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