Application | Comment | Organism |
---|---|---|
synthesis | - |
Physarum polycephalum |
synthesis | synthesis of steryl beta-D-monoglucosides that are constituents of the cell membrane of higher plants | Sinapis alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coprostanol | potent inhibitor | Physarum polycephalum | |
coprostanol | not inhibitory | Sinapis alba | |
epicholestanol | potent inhibitor | Physarum polycephalum | |
epicholestanol | not inhibitory | Sinapis alba | |
sodium deoxycholate | - |
Sinapis alba | |
sodium taurocholate | - |
Sinapis alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
sitosterol | - |
Sinapis alba | |
0.08 | - |
UDP-glucose | - |
Sinapis alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Physarum polycephalum | 16020 | - |
membrane | organelles membrane | Sinapis alba | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Physarum polycephalum | |
Ca2+ | not stimulated between 0.001 mM to 10 mM | Sinapis alba | |
Hg2+ | - |
Physarum polycephalum | |
Hg2+ | 50% of inhibition at 0.4 mM | Sinapis alba | |
Mg2+ | - |
Physarum polycephalum | |
Mg2+ | not stimulated between 0.001 mM to 10 mM | Sinapis alba | |
Zn2+ | - |
Physarum polycephalum | |
Zn2+ | 50% of inhibition at 0.006 mM | Sinapis alba |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
by gel filtration | Sinapis alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Sinapis alba | - |
white mustard | - |
Purification (Comment) | Organism |
---|---|
partial purification by differential centrifugation and Sephadex G-150 column | Sinapis alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | mycoplasma strain M3C IV | Physarum polycephalum | - |
seedling | - |
Sinapis alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is absolute specific to UDP-glucose | Sinapis alba | ? | - |
? | |
additional information | glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates | Physarum polycephalum | ? | - |
? | |
additional information | glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates | Sinapis alba | ? | - |
? | |
additional information | enzyme can use CDP-glucose and TDP-glucose at 6 times slower rate | Physarum polycephalum | ? | - |
? | |
additional information | coprostanol and epicholestanol are not glucosylated | Physarum polycephalum | ? | - |
? | |
additional information | all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation | Physarum polycephalum | ? | - |
? | |
additional information | all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation | Sinapis alba | ? | - |
? | |
UDP-glucose + campesterol | - |
Physarum polycephalum | UDP + campesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + campesterol | best substrate | Sinapis alba | UDP + campesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | - |
Sinapis alba | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | best substrate | Physarum polycephalum | UDP + sitosterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Physarum polycephalum |
30 | - |
assay at | Sinapis alba |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
Tris-HCl 0.1 M, Triton X-100 0.3% | Sinapis alba |