Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10% stimulation at 1 mM | Physarum polycephalum | |
ATP | 15% stimulation at 0.01 mM | Physarum polycephalum | |
dithiothreitol | 25% stimulation at 1 mM | Physarum polycephalum | |
EDTA | 15% stimulation at 0.1 mM | Physarum polycephalum | |
lecithin | 20-25% stimulation | Physarum polycephalum |
Application | Comment | Organism |
---|---|---|
synthesis | synthesis of steryl beta-D-monoglucosides possible function as glucose carriers through the membrane and intercellular transport of sterols | Physarum polycephalum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 50% inhibition at 0.60 mM | Physarum polycephalum | |
Triton X-100 | strong inhibition at concentrations below 0.05% | Physarum polycephalum | |
Tween 20 | strong inhibition at concentrations below 0.05% | Physarum polycephalum | |
Tween 60 | strong inhibition at concentrations below 0.05% | Physarum polycephalum | |
UDP | 50% inhibition at 0.06 mM | Physarum polycephalum | |
UTP | 50% inhibition at 0.70 mM | Physarum polycephalum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
sitosterol | - |
Physarum polycephalum | |
0.18 | - |
UDP-glucose | - |
Physarum polycephalum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 50% of the activity | Physarum polycephalum | 5737 | - |
membrane | membrane bound 50% of the activity | Physarum polycephalum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly inhibition above 0.1 mM | Physarum polycephalum | |
Hg2+ | 50% inhibition at 0.07 mM | Physarum polycephalum | |
Mg2+ | slightly inhibition above 0.1 mM | Physarum polycephalum | |
Zn2+ | 50% inhibition at 0.05 mM | Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by differential centrifugation and gel filtration on Sephadex G-100 | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | mycoplasma strain M3C IV | Physarum polycephalum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DELTA5-sterols are glucosylated at higher rate than DELTA7-sterols | Physarum polycephalum | ? | - |
? | |
additional information | presence and localization of double bonds in the ring system exert a pronounced effect on the rate of glucosylation | Physarum polycephalum | ? | - |
? | |
additional information | the presence of a DELTA22 double bond decreases affinity of the sterol for the enzyme | Physarum polycephalum | ? | - |
? | |
additional information | sterols possessing an alkyl group at C-24 are better substrates than C27 sterols | Physarum polycephalum | ? | - |
? | |
additional information | stanols and sterols with conjugated double bonds in ring B are poor substrates | Physarum polycephalum | ? | - |
? | |
UDP-glucose + 5-alpha-cholestanol | 8% of the activity with sitosterol | Physarum polycephalum | UDP + 5-alpha-cholestanol 3-beta-D-glucoside | - |
? | |
UDP-glucose + 5-cholestenol | 12% of the activity with sitosterol | Physarum polycephalum | UDP + 5-cholestenol 3-beta-D-glucoside | - |
? | |
UDP-glucose + 7-dehydrocholesterol | 6% of the activity with sitosterol | Physarum polycephalum | UDP + 7-dehydrocholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + alpha-spinasterol | 31% of the activity with sitosterol | Physarum polycephalum | UDP + alpha-spinasterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + campesterol | 28% of the activity with sitosterol | Physarum polycephalum | UDP + campesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | 23% of the activity with sitosterol | Physarum polycephalum | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + ergosterol | 11% of the activity with sitosterol | Physarum polycephalum | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + sitosterol | greater activity than with other sterols | Physarum polycephalum | UDP + sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | 50% of the activity with sitosterol | Physarum polycephalum | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Physarum polycephalum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
Tris-HCl 0.1 M | Physarum polycephalum |