Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.173 extracted from

  • Wojciechowski, J.; Zimowski, J.; Tyski, S.
    Enzymatic synthesis of steryl 3-beta-D-monoglucosides in the slime mold Physarum polycephalum (1977), Phytochemistry, 16, 911-914.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10% stimulation at 1 mM Physarum polycephalum
ATP 15% stimulation at 0.01 mM Physarum polycephalum
dithiothreitol 25% stimulation at 1 mM Physarum polycephalum
EDTA 15% stimulation at 0.1 mM Physarum polycephalum
lecithin 20-25% stimulation Physarum polycephalum

Application

Application Comment Organism
synthesis synthesis of steryl beta-D-monoglucosides possible function as glucose carriers through the membrane and intercellular transport of sterols Physarum polycephalum

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 50% inhibition at 0.60 mM Physarum polycephalum
Triton X-100 strong inhibition at concentrations below 0.05% Physarum polycephalum
Tween 20 strong inhibition at concentrations below 0.05% Physarum polycephalum
Tween 60 strong inhibition at concentrations below 0.05% Physarum polycephalum
UDP 50% inhibition at 0.06 mM Physarum polycephalum
UTP 50% inhibition at 0.70 mM Physarum polycephalum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
sitosterol
-
Physarum polycephalum
0.18
-
UDP-glucose
-
Physarum polycephalum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm 50% of the activity Physarum polycephalum 5737
-
membrane membrane bound 50% of the activity Physarum polycephalum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly inhibition above 0.1 mM Physarum polycephalum
Hg2+ 50% inhibition at 0.07 mM Physarum polycephalum
Mg2+ slightly inhibition above 0.1 mM Physarum polycephalum
Zn2+ 50% inhibition at 0.05 mM Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
gel filtration Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and gel filtration on Sephadex G-100 Physarum polycephalum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture mycoplasma strain M3C IV Physarum polycephalum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DELTA5-sterols are glucosylated at higher rate than DELTA7-sterols Physarum polycephalum ?
-
?
additional information presence and localization of double bonds in the ring system exert a pronounced effect on the rate of glucosylation Physarum polycephalum ?
-
?
additional information the presence of a DELTA22 double bond decreases affinity of the sterol for the enzyme Physarum polycephalum ?
-
?
additional information sterols possessing an alkyl group at C-24 are better substrates than C27 sterols Physarum polycephalum ?
-
?
additional information stanols and sterols with conjugated double bonds in ring B are poor substrates Physarum polycephalum ?
-
?
UDP-glucose + 5-alpha-cholestanol 8% of the activity with sitosterol Physarum polycephalum UDP + 5-alpha-cholestanol 3-beta-D-glucoside
-
?
UDP-glucose + 5-cholestenol 12% of the activity with sitosterol Physarum polycephalum UDP + 5-cholestenol 3-beta-D-glucoside
-
?
UDP-glucose + 7-dehydrocholesterol 6% of the activity with sitosterol Physarum polycephalum UDP + 7-dehydrocholesterol 3-beta-D-glucoside
-
?
UDP-glucose + alpha-spinasterol 31% of the activity with sitosterol Physarum polycephalum UDP + alpha-spinasterol 3-beta-D-glucoside
-
?
UDP-glucose + campesterol 28% of the activity with sitosterol Physarum polycephalum UDP + campesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol 23% of the activity with sitosterol Physarum polycephalum UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol 11% of the activity with sitosterol Physarum polycephalum UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol greater activity than with other sterols Physarum polycephalum UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol 50% of the activity with sitosterol Physarum polycephalum UDP + stigmasterol 3-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Physarum polycephalum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2
-
Tris-HCl 0.1 M Physarum polycephalum