Literature summary for 2.4.1.173 extracted from
Kalinowska, M.; Wojciechowski, Z.A.
Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids (1986), Phytochemistry, 25, 45-49.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
Phospholipids |
not stimulatory |
Sinapis alba |
|
Triton X-100 |
activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents |
Sinapis alba |
|
Tween-20 |
2fold activity stimulation at 0.1% |
Sinapis alba |
|
Tween-40 |
70% activity stimulation at 0.05% |
Sinapis alba |
|
Tween-60 |
35% activity stimulation at 0.1% |
Sinapis alba |
|
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
sodium deoxycholate |
strong inhibition at 0.1-1% |
Sinapis alba |
|
sodium taurocholate |
strong inhibition at 0.1-1% |
Sinapis alba |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
membrane |
organelles membrane |
Sinapis alba |
16020 |
- |
Organism
Organism |
UniProt |
Comment |
Textmining |
Sinapis alba |
- |
white mustard |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
partial purification by differential centrifugation and precipitation with Me2CO |
Sinapis alba |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
seedling |
- |
Sinapis alba |
- |
Storage Stability
Storage Stability |
Organism |
-20 C, Tris-HCl 0.1 M, pH 7.2, two weeks |
Sinapis alba |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
UDP-glucose + cholesterol |
- |
Sinapis alba |
UDP + cholesterol 3-beta-D-glucoside |
- |
? |
|
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
30 |
- |
assay at |
Sinapis alba |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
7.2 |
- |
Tris-HCl 0.1 M |
Sinapis alba |