BRENDA - Enzyme Database
show all sequences of 2.4.1.172

Partial purification and characterization of UDP-glucose:salicyl alcohol glucosyltransferase from Gardenia jasminoides cell cultures

Mizukami, H.; Terao, T.; Ohashi, H.; Planta Med. 1985, 104-107 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
2 mM, 80% inhibition
Gardenia jasminoides
Mn2+
2 mM, 20% inhibition
Gardenia jasminoides
N-ethylmaleimide
10 mM, 35% inhibition
Gardenia jasminoides
p-chloromercuribenzoate
10 mM, 25% inhibition
Gardenia jasminoides
Zn2+
2 mM, 60% inhibition
Gardenia jasminoides
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
UDPglucose
-
Gardenia jasminoides
0.11
-
salicyl alcohol
-
Gardenia jasminoides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
gel filtration
Gardenia jasminoides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPglucose + salicyl alcohol
Gardenia jasminoides
-
UDP + salicin
-
-
?
UDPglucose + salicyl alcohol
Gardenia jasminoides Ellis
-
UDP + salicin
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Gardenia jasminoides
-
Ellis
-
Gardenia jasminoides Ellis
-
Ellis
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Gardenia jasminoides
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
cell culture
Gardenia jasminoides
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.165
-
-
Gardenia jasminoides
Storage Stability
Storage Stability
Organism
4°C, 2 weeks, 40% loss of activity, or -20°C, 2 weeks, 40% loss of activity
Gardenia jasminoides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides
UDP + salicin
-
-
-
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides
UDP + salicin
-
488805
Gardenia jasminoides
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides Ellis
UDP + salicin
-
-
-
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides Ellis
UDP + salicin
-
488805
Gardenia jasminoides Ellis
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Gardenia jasminoides
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, 10% remaining activity
Gardenia jasminoides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Gardenia jasminoides
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
45% of maximal activity at pH 6.0, 10% of maximal activity at pH 10.5
Gardenia jasminoides
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
stable
Gardenia jasminoides
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
2 mM, 80% inhibition
Gardenia jasminoides
Mn2+
2 mM, 20% inhibition
Gardenia jasminoides
N-ethylmaleimide
10 mM, 35% inhibition
Gardenia jasminoides
p-chloromercuribenzoate
10 mM, 25% inhibition
Gardenia jasminoides
Zn2+
2 mM, 60% inhibition
Gardenia jasminoides
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
UDPglucose
-
Gardenia jasminoides
0.11
-
salicyl alcohol
-
Gardenia jasminoides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
51000
-
gel filtration
Gardenia jasminoides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPglucose + salicyl alcohol
Gardenia jasminoides
-
UDP + salicin
-
-
?
UDPglucose + salicyl alcohol
Gardenia jasminoides Ellis
-
UDP + salicin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Gardenia jasminoides
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
cell culture
Gardenia jasminoides
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.165
-
-
Gardenia jasminoides
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 2 weeks, 40% loss of activity, or -20°C, 2 weeks, 40% loss of activity
Gardenia jasminoides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides
UDP + salicin
-
-
-
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides
UDP + salicin
-
488805
Gardenia jasminoides
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides Ellis
UDP + salicin
-
-
-
?
UDPglucose + salicyl alcohol
-
488805
Gardenia jasminoides Ellis
UDP + salicin
-
488805
Gardenia jasminoides Ellis
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Gardenia jasminoides
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, 10% remaining activity
Gardenia jasminoides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Gardenia jasminoides
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
45% of maximal activity at pH 6.0, 10% of maximal activity at pH 10.5
Gardenia jasminoides
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
9
stable
Gardenia jasminoides
Other publictions for EC 2.4.1.172
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
488805
Mizukami
-
Partial purification and chara ...
Gardenia jasminoides, Gardenia jasminoides Ellis
Planta Med.
1985
104-107
1985
-
-
-
-
-
-
5
2
-
-
1
2
-
2
-
-
1
-
-
1
1
1
4
-
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
-
1
2
-
-
-
1
-
1
1
1
4
-
1
-
1
-
1
1
1
-
-
-
-
-
-
-
488806
Terao
-
Position-specific glucosylatio ...
Gardenia jasminoides
Plant Sci. Lett.
33
47-52
1984
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
1
-
1
2
-
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
1
-
1
2
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-