Cloned (Comment) | Organism |
---|---|
expressed in HEK-293 and CHO cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0457 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.0569 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens | |
0.554 | - |
entacapone | with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.703 | - |
entacapone | without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
7.3 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
10.4 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM used in assay conditions | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisPur cobalt resin column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastrointestinal tract | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
entacapone + UDP-glucuronate | - |
Homo sapiens | UDP + entacapone-3-O-glucuronide | - |
? | |
morphine + UDP-glucuronate | - |
Homo sapiens | UDP + morphine-3-glucuronide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, His6-tagged recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase | - |
Homo sapiens |
UGT1A10 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
entacapone | without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.0027 | - |
entacapone | with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.015 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens | |
0.073 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens | |
0.138 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.422 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00144 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens | |
0.00187 | - |
entacapone | without phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.0048 | - |
entacapone | with phospholipid purified recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
0.0188 | - |
morphine | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens | |
1.285 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from HEK-293 cells, at pH 7.4 and 37°C | Homo sapiens | |
9.22 | - |
entacapone | membrane-bound recombinant isoform UGT1A10 from CHO cells, at pH 7.4 and 37°C | Homo sapiens |