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Literature summary for 2.4.1.17 extracted from

  • Lewis, B.C.; Mackenzie, P.I.; Elliot, D.J.; Burchell, B.; Bhasker, C.R.; Miners, J.O.
    Amino terminal domains of human UDP-glucuronosyltransferases (UGT) 2B7 and 2B15 associated with substrate selectivity and autoactivation (2007), Biochem. Pharmacol., 73, 1463-1473.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type isozyme UGR2B15, and of chimeric mutants in HEK-293 cells Homo sapiens
expression of wild-type isozyme UGT2B7, and of chimeric mutants in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of UGT2B7–UGT2B15 chimeras, e.g. a UGT2B7-15-7 chimera that incorporated amino acids 61-194 of UGT2B15 glucuronidates the UGT2B15 substrates testosterone and phenolphthalein, but not the UGT2B7 substrates zidovudine and 11a-hydroxyprogesterone. UGT2B7-15(61-157)-7, UGT2B7-15(91-157)-7 and UGT2B7-15(61-91)-7 glucuronidate 4-methylumbelliferone, but activity towards the other substrates is not detected. Like UGT2B7, the UGT2B7-15(61-157)-7, UGT2B7-15(91-157)-7 and UGT2B7-15(61-91)-7 chimeras exhibit sigmoidal 4-methylumbelliferone glucuronidation kinetics, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information glucuronidation of the non-selective substrate 4-methylumbelliferone by mutant chimeric UGT2B7-15(61–194)-7 and isozyme UGT2B15 followed Michaelis–Menten and weak substrate inhibition kinetics, respectively, overview Homo sapiens
additional information
-
additional information glucuronidation of the non-selective substrate 4-methylumbelliferone by mutant chimeric UGT2B7-15(61–194)-7 follows Michaelis–Menten and weak substrate inhibition kinetics, respectively, whereas 4-methylumbelliferone glucuronidation by isozyme UGT2B7 exhibited sigmoidal kinetics characteristic of autoactivation, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-glucuronate + acceptor Homo sapiens
-
UDP + acceptor beta-D-glucuronoside
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16662 isozyme UGT2B7; isozyme UGT2B7
-
Homo sapiens P54855 isozyme UGT2B15; isozyme UGR2B15
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of isozyme UGT2B15, overview Homo sapiens ?
-
?
additional information substrate specificity of isozyme UGT2B7, overview Homo sapiens ?
-
?
UDP-D-glucuronate + 11alpha-hydroxyprogesterone an UGT2B7 substrate Homo sapiens UDP + 11alpha-hydroxyprogesterone 11-O-beta-D-glucuronoside
-
?
UDP-D-glucuronate + 4-methylumbelliferone non-selective substrate Homo sapiens UDP + 4-methylumbelliferone beta-D-glucuronoside
-
?
UDP-D-glucuronate + acceptor
-
Homo sapiens UDP + acceptor beta-D-glucuronoside
-
?
UDP-D-glucuronate + phenolphthalein an UGT2B15 substrate Homo sapiens UDP + phenolphthalein beta-D-glucuronoside
-
?
UDP-D-glucuronate + testosterone an UGT2B15 substrate Homo sapiens UDP + testosterone 17-O-beta-D-glucuronoside
-
?
UDP-D-glucuronate + zidovudine an UGT2B7 substrate Homo sapiens UDP + zidovudine beta-D-glucuronoside
-
?

Synonyms

Synonyms Comment Organism
UDP-glucuronosyltransferases 2B15
-
Homo sapiens
UDP-glucuronosyltransferases 2B7
-
Homo sapiens
UGT2B15
-
Homo sapiens
UGT2B7
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens