Cloned (Comment) | Organism |
---|---|
expression of wild-type isozyme UGR2B15, and of chimeric mutants in HEK-293 cells | Homo sapiens |
expression of wild-type isozyme UGT2B7, and of chimeric mutants in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of UGT2B7UGT2B15 chimeras, e.g. a UGT2B7-15-7 chimera that incorporated amino acids 61-194 of UGT2B15 glucuronidates the UGT2B15 substrates testosterone and phenolphthalein, but not the UGT2B7 substrates zidovudine and 11a-hydroxyprogesterone. UGT2B7-15(61-157)-7, UGT2B7-15(91-157)-7 and UGT2B7-15(61-91)-7 glucuronidate 4-methylumbelliferone, but activity towards the other substrates is not detected. Like UGT2B7, the UGT2B7-15(61-157)-7, UGT2B7-15(91-157)-7 and UGT2B7-15(61-91)-7 chimeras exhibit sigmoidal 4-methylumbelliferone glucuronidation kinetics, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | glucuronidation of the non-selective substrate 4-methylumbelliferone by mutant chimeric UGT2B7-15(61194)-7 and isozyme UGT2B15 followed MichaelisMenten and weak substrate inhibition kinetics, respectively, overview | Homo sapiens | |
additional information | - |
additional information | glucuronidation of the non-selective substrate 4-methylumbelliferone by mutant chimeric UGT2B7-15(61194)-7 follows MichaelisMenten and weak substrate inhibition kinetics, respectively, whereas 4-methylumbelliferone glucuronidation by isozyme UGT2B7 exhibited sigmoidal kinetics characteristic of autoactivation, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate + acceptor | Homo sapiens | - |
UDP + acceptor beta-D-glucuronoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16662 | isozyme UGT2B7; isozyme UGT2B7 | - |
Homo sapiens | P54855 | isozyme UGT2B15; isozyme UGR2B15 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity of isozyme UGT2B15, overview | Homo sapiens | ? | - |
? | |
additional information | substrate specificity of isozyme UGT2B7, overview | Homo sapiens | ? | - |
? | |
UDP-D-glucuronate + 11alpha-hydroxyprogesterone | an UGT2B7 substrate | Homo sapiens | UDP + 11alpha-hydroxyprogesterone 11-O-beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + 4-methylumbelliferone | non-selective substrate | Homo sapiens | UDP + 4-methylumbelliferone beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + acceptor | - |
Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + phenolphthalein | an UGT2B15 substrate | Homo sapiens | UDP + phenolphthalein beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + testosterone | an UGT2B15 substrate | Homo sapiens | UDP + testosterone 17-O-beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + zidovudine | an UGT2B7 substrate | Homo sapiens | UDP + zidovudine beta-D-glucuronoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferases 2B15 | - |
Homo sapiens |
UDP-glucuronosyltransferases 2B7 | - |
Homo sapiens |
UGT2B15 | - |
Homo sapiens |
UGT2B7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |