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Literature summary for 2.4.1.17 extracted from

  • Takeda, S.; Ishii, Y.; Iwanaga, M.; Mackenzie, P.I.; Nagata, K.; Yamazoe, Y.; Oguri, K.; Yamada, H.
    Modulation of UDP-glucuronosyltransferase function by cytochrome P450: evidence for the alteration of UGT2B7-catalyzed glucuronidation of morphine by CYP3A4 (2005), Mol. Pharmacol., 67, 665-672.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of isozyme UGT2B7 with either CYP3A4, CYP1A2 or CYP2C9 cytochrome P450 enzymes in COS-1 cells, coexpression alters the kinetic properties of isozyme UGT2B7 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
morphine isozyme UGT2B7 in COS-1 cell microsomes, pH 7.8, 37°C, in presence with CYP2C9 Homo sapiens
0.38
-
morphine isozyme UGT2B7 in COS-1 cell microsomes, pH 7.8, 37°C Homo sapiens
0.48
-
morphine isozyme UGT2B7 in COS-1 cell microsomes, pH 7.8, 37°C, in presence with CYP1A2 Homo sapiens
1.18
-
morphine liver microsomes, pH 7.8, 37°C Homo sapiens
3.71
-
morphine isozyme UGT2B7 in COS-1 cell microsomes, pH 7.8, 37°C, in presence with CYP3A4 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronate + morphine Homo sapiens regioselectivity of activity is altered by cytochrome P450 enzyme CYP3A4 so that the ratio of morphine activation/detoxification is increased UDP + morphine-3-beta-D-glucuronide
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P16662 isozyme UGT2B7; isozyme UGT2B7
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
999
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UGT2B7 interacts with CYP3A4, the latter reduces UGT2B7 activity in detergent n-octylglucoside treated recombinant COS-cell microsomes Homo sapiens ?
-
?
UDP-glucuronate + morphine regioselectivity of activity is altered by cytochrome P450 enzyme CYP3A4 so that the ratio of morphine activation/detoxification is increased Homo sapiens UDP + morphine-3-beta-D-glucuronide
-
?
UDP-glucuronate + morphine regioselective Homo sapiens UDP + morphine-3-beta-D-glucuronide
-
?

Synonyms

Synonyms Comment Organism
UDP-glucuronosyltransferase
-
Homo sapiens
UGT
-
Homo sapiens
UGT2B7
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens