Cloned (Comment) | Organism |
---|---|
isozyme UGT1A4, DNA and amino acid sequence determination and analysis, expression of isozyme UGT1A4 in COS-7 cells, analysis of polymorphisms naturally occuring in a Japanese population | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L48V | naturally occuring polymorphism, increased activity with trans-androsterone, imipramine, and cyproheptadine, less active with tigogenin as compared to the wild-type enzyme | Homo sapiens |
additional information | isozyme UGT1A4 polymorphisms in a Japanese population, identification of 3 single nucleotides polymorphisms in exon 1, 1 in intron 1, and of 3 types of alleles with distinct SNP combinations, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
tigogenin | recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C | Homo sapiens | |
0.008 | - |
trans-androsterone | recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C | Homo sapiens | |
0.008 | - |
tigogenin | recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C | Homo sapiens | |
0.025 | - |
clozapine | recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C | Homo sapiens | |
0.049 | - |
clozapine | recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C | Homo sapiens | |
0.086 | - |
cyproheptadine | recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C | Homo sapiens | |
0.228 | - |
cyproheptadine | recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C | Homo sapiens | |
0.255 | - |
trans-androsterone | recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C | Homo sapiens | |
0.276 | - |
imipramine | recombinant mutant isozyme UGT1A4 L48V, pH 8.4, 37°C | Homo sapiens | |
1.442 | - |
imipramine | recombinant wild-type isozyme UGT1A4, pH 8.4, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, recombinant isozyme UGT1A4, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22310 | isozyme UGT1A4; isozyme UGT1A4 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + clozapine | - |
Homo sapiens | UDP + clozapine beta-D-glucuronide | - |
? | |
UDP-glucuronate + cyproheptadine | - |
Homo sapiens | UDP + cyproheptadine beta-D-glucuronide | - |
? | |
UDP-glucuronate + imipramine | - |
Homo sapiens | UDP + imipramine beta-D-glucuronide | - |
? | |
UDP-glucuronate + tigogenin | - |
Homo sapiens | UDP + tigogenin beta-D-glucuronide | - |
? | |
UDP-glucuronate + trans-androsterone | - |
Homo sapiens | UDP + trans-androsterone beta-D-glucuronide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant isozyme UGT1A4, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase 1A4 | - |
Homo sapiens |
UGT1A4 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Homo sapiens |