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Literature summary for 2.4.1.17 extracted from

  • Iwano, H.; Yokota, H.; Ohgiya, S.; Yuasa, A.
    The significance of amino acid residue Asp446 for enzymic stability of rat UDP-glucuronosyltransferase UGT1A6 (1999), Arch. Biochem. Biophys., 363, 116-120.
    View publication on PubMed
Search for more literature for 2.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast AH22 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D446A UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446E UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446G UGT1A6 activity not detected for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446K UGT1A6 activity not detected for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446N UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446q UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
D446T UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00246
-
 1-naphthol apparent Km for UGT1A6, 3mM UDP-glucuronic acid, mutants that retained activity shows similar Km values Rattus norvegicus
0.26
-
 UDP-glucuronic acid apparent Km for UGT1A6, 50mM 1-naphthol, mutants that retained activity shows similar Km values Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
endoplasmic reticulum
-
 Rattus norvegicus 5783
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 UGT1A6 wild-type and all its mutants Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthol + UDP-glucuronate
-
 Homo sapiens naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
1-naphthol + UDP-glucuronate
-
 Rattus norvegicus naphth-1-yl-beta-D-glucuronide + UDP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 wild type UGT1A6 stable for 30 min, mutants inactives after preincubation Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 UGT1A6 mutants activity reduced at acidic pH Rattus norvegicus