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Literature summary for 2.4.1.17 extracted from
- Steroid glucuronyltransferases. II. Solubilization, purification aand kinetic properties of oestradiol-17 3-glucuronyltransferase from the microsomal fraction of pig intestine (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1223-1230.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| estradiol | - |
Sus scrofa |  |
| estrone | competitive to estradiol-17beta | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Sus scrofa | |
| 0.046 | - |
estradiol-17beta | range between 0.045 mM and 0.047 mM | Sus scrofa | |
| 0.22 | - |
UDPglucuronic acid | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Sus scrofa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
UGT2B7 | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| small intestine | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable | Sus scrofa |
| 0°C, 14 days, 80% loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| estradiol-17alpha + UDPglucuronate | - |
Sus scrofa | UDP + 17-alpha-estradiol O-glucuronide | - |
? | |
| estradiol-17beta + UDPglucuronate | - |
Sus scrofa | estradiol-17beta-3-monoglucuronide + UDP | - |
? | |
| estriol + UDPglucuronate | - |
Sus scrofa | UDP + estriol-16 alpha-glucuronide | - |
? | |
| estrone + UDPglucuronate | - |
Sus scrofa | UDP + estrone 3-O-glucuronide | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
increase of activity up to 50°C, half-life at 50°C: 15 min | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable up to | Sus scrofa |
| 50 | - |
half-life 15 min | Sus scrofa |
| 62 | - |
complete inactivation | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
estradiol-17-beta, Tris/maleate or phosphate buffer | Sus scrofa |
| 7.6 | - |
estradiol-17beta as substrate, Tris/HCl buffer | Sus scrofa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
estradiol | - |
Sus scrofa | |
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