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Literature summary for 2.4.1.17 extracted from

  • Rao, M.L.; Rao, G.S.; Breuer, H.
    Investigations on the kinetic properties of estrone glucuronyltransferase from pig kidney (1976), Biochim. Biophys. Acta, 452, 89-100.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Sus scrofa
bilirubin linear noncompetitive inhibition towards estrone Sus scrofa
Cd2+ complete inactivation at 10 mM Sus scrofa
Cu2+ 10 mM, complete inactivation Sus scrofa
EDTA not inhibited Sus scrofa
estradiol 17alpha or 17beta, noncompetitive inhibition towards estrone Sus scrofa
estriol linear noncompetitive inhibition towards estrone Sus scrofa
Hg2+ complete inactivation Sus scrofa
additional information dithioerythritol; not inhibitory: N-ethylmaleimide; product inhibition Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa
Phenolphthalein linear noncompetitive inhibition towards estrone Sus scrofa
testosterone linear noncompetitive inhibition towards estrone Sus scrofa
UDP noncompetitive inhibition towards UDPglucuronic acid at saturating concentration of UDPglucuronic acid, linear and uncompetitive inhibition with saturating concentration of estrone and varying concentration of UDPglucuronic acid Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
estrone
-
Sus scrofa
0.025
-
UDPglucuronic acid
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, 30% activation Sus scrofa
Mg2+ 10 mM, 30% activation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
-20°C, microsomal preparation, more than 4 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
estrone + UDPglucuronate affinity increases during storage period at -20ºC for more than 4 months Sus scrofa UDP + estrone 3-O-glucuronide
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2 45 linear estrone glucuronide formation Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
15 min, absence of substrates, slight inactivation Sus scrofa
48
-
15 min, 50% loss of activity Sus scrofa
70
-
15 min, complete inactivation Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 8.7 estrone Sus scrofa