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Literature summary for 2.4.1.17 extracted from

  • Bock, K.W.; Josting, D.; Lilienblum, W.; Pfeil, H.
    Purification of rat-liver microsomal UDP-glucuronyltransferase. Separation of two enzyme forms inducible by 3-methylcholanthrene or phenobarbital (1979), Eur. J. Biochem., 98, 19-26.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-methylcholanthrene several-fold activity enhanced of 1-naphthol, 4-nitrophenol, N-hydroxy-2-naphtylamine and 3-hydroxy benzo[a]pyrene Rattus norvegicus
lysophosphatidylcholine slight stimulation of highly purified preparations Rattus norvegicus
Phenobarbital stimulates action against 4-hydroxybiphenyl, chloramphenicol and bilirubin Rattus norvegicus
UDP-N-acetylglucosamine no effect on the purified enzyme Rattus norvegicus
UDP-N-acetylglucosamine physiological activator of UDPGT in the latent membrane-bond state of the enzyme without effect on the purified enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
UDP
-
Rattus norvegicus
UDPgalacturonic acid slight effect Rattus norvegicus
UDPglucose slight effect Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2fold activation of purified enzyme Rattus norvegicus
Mg2+ as MgCl2: 5 mM, activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
1-naphthol-UDPGT by SDS-PAGE Rattus norvegicus
56000
-
x * 56000, phenobarbital-inducible isozyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Wistar strain
-
Rattus norvegicus Wistar
-
Wistar strain
-

Purification (Commentary)

Purification (Comment) Organism
purification of two isozymes inducibles by 3-methylcholanthrene or phenobarbital using sodium cholate solubilization, chromatography on Bio Gel A 1.5 m, DEAE-cellulose and UDP-hexanolamine Sepharose 4B Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
lung
-
Rattus norvegicus
-
skin
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
comparison of activities between differentially inducible forms Rattus norvegicus
0.67
-
morphine as substrate, Wistar strain treated with phenobarbital Rattus norvegicus
5.35
-
1-naphthol as substrate, Wistar strain treated with phenobarbital Rattus norvegicus
6.06
-
1-naphthol as substrate, Wistar strain treated with 3-methylcholanthrene Rattus norvegicus

Storage Stability

Storage Stability Organism
0-4°C, 0.1 mg/ml lysophosphatidylcholine, 1 mM EDTA, 11% loss of activity in 6 days, 20% loss of activity in 12 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthol + UDP-glucuronate
-
Rattus norvegicus naphth-1-yl-beta-D-glucuronide + UDP
-
?
1-naphthol + UDP-glucuronate
-
Rattus norvegicus Wistar naphth-1-yl-beta-D-glucuronide + UDP
-
?
3-hydroxybenzo[a]pyrene + UDPglucuronate
-
Rattus norvegicus UDP + 3-hydroxybenzo[a]pyrene-O-glucuronide
-
?
4-hydroxybiphenyl + UDP-glucuronate
-
Rattus norvegicus UDP + 4-hydroxybiphenyl 4-O-beta-D-glucuronide
-
?
4-nitrophenol + UDP-glucuronate
-
Rattus norvegicus 4-nitrophenyl beta-D-glucuronide + UDP
-
?
4-nitrophenol + UDP-glucuronate
-
Rattus norvegicus Wistar 4-nitrophenyl beta-D-glucuronide + UDP
-
?
estrone + UDPglucuronate
-
Rattus norvegicus UDP + estrone 3-O-glucuronide
-
?
additional information specificity not influenced by lysophosphatidylcholine Rattus norvegicus ?
-
?
additional information specificity not influenced by lysophosphatidylcholine Rattus norvegicus Wistar ?
-
?
morphine + UDP-glucuronate
-
Rattus norvegicus UDP + morphine 3,6-diglucuronide
-
?
morphine + UDP-glucuronate
-
Rattus norvegicus Wistar UDP + morphine 3,6-diglucuronide
-
?
N-hydroxy-2-naphthylamine + UDPglucuronate
-
Rattus norvegicus UDP + N-hydroxy-2-naphthylamine-O-glucuronide
-
?
N-hydroxy-2-naphthylamine + UDPglucuronate
-
Rattus norvegicus Wistar UDP + N-hydroxy-2-naphthylamine-O-glucuronide
-
?
testosterone + UDPglucuronate
-
Rattus norvegicus UDP + testosterone O-glucuronide
-
?
UDP-alpha-D-glucuronate + chloramphenicol
-
Rattus norvegicus UDP + chloramphenicol beta-D-glucuronide
-
?

Subunits

Subunits Comment Organism
? x * 56000, phenobarbital-inducible isozyme Rattus norvegicus
? additional values for rat isozymes ranging from 51000 to 59000 Rattus norvegicus
? x * 54000, methylcholanthrene-inducible isozyme Rattus norvegicus
? all values determined by SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
rapid loss of activity Rattus norvegicus