Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-methylcholanthrene | several-fold activity enhanced of 1-naphthol, 4-nitrophenol, N-hydroxy-2-naphtylamine and 3-hydroxy benzo[a]pyrene | Rattus norvegicus | |
lysophosphatidylcholine | slight stimulation of highly purified preparations | Rattus norvegicus | |
Phenobarbital | stimulates action against 4-hydroxybiphenyl, chloramphenicol and bilirubin | Rattus norvegicus | |
UDP-N-acetylglucosamine | no effect on the purified enzyme | Rattus norvegicus | |
UDP-N-acetylglucosamine | physiological activator of UDPGT in the latent membrane-bond state of the enzyme without effect on the purified enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | - |
Rattus norvegicus | |
UDPgalacturonic acid | slight effect | Rattus norvegicus | |
UDPglucose | slight effect | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2fold activation of purified enzyme | Rattus norvegicus | |
Mg2+ | as MgCl2: 5 mM, activation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1-naphthol-UDPGT by SDS-PAGE | Rattus norvegicus |
56000 | - |
x * 56000, phenobarbital-inducible isozyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar strain | - |
Rattus norvegicus Wistar | - |
Wistar strain | - |
Purification (Comment) | Organism |
---|---|
purification of two isozymes inducibles by 3-methylcholanthrene or phenobarbital using sodium cholate solubilization, chromatography on Bio Gel A 1.5 m, DEAE-cellulose and UDP-hexanolamine Sepharose 4B | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
skin | - |
Rattus norvegicus | - |
small intestine | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of activities between differentially inducible forms | Rattus norvegicus |
0.67 | - |
morphine as substrate, Wistar strain treated with phenobarbital | Rattus norvegicus |
5.35 | - |
1-naphthol as substrate, Wistar strain treated with phenobarbital | Rattus norvegicus |
6.06 | - |
1-naphthol as substrate, Wistar strain treated with 3-methylcholanthrene | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0-4°C, 0.1 mg/ml lysophosphatidylcholine, 1 mM EDTA, 11% loss of activity in 6 days, 20% loss of activity in 12 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthol + UDP-glucuronate | - |
Rattus norvegicus | naphth-1-yl-beta-D-glucuronide + UDP | - |
? | |
1-naphthol + UDP-glucuronate | - |
Rattus norvegicus Wistar | naphth-1-yl-beta-D-glucuronide + UDP | - |
? | |
3-hydroxybenzo[a]pyrene + UDPglucuronate | - |
Rattus norvegicus | UDP + 3-hydroxybenzo[a]pyrene-O-glucuronide | - |
? | |
4-hydroxybiphenyl + UDP-glucuronate | - |
Rattus norvegicus | UDP + 4-hydroxybiphenyl 4-O-beta-D-glucuronide | - |
? | |
4-nitrophenol + UDP-glucuronate | - |
Rattus norvegicus | 4-nitrophenyl beta-D-glucuronide + UDP | - |
? | |
4-nitrophenol + UDP-glucuronate | - |
Rattus norvegicus Wistar | 4-nitrophenyl beta-D-glucuronide + UDP | - |
? | |
estrone + UDPglucuronate | - |
Rattus norvegicus | UDP + estrone 3-O-glucuronide | - |
? | |
additional information | specificity not influenced by lysophosphatidylcholine | Rattus norvegicus | ? | - |
? | |
additional information | specificity not influenced by lysophosphatidylcholine | Rattus norvegicus Wistar | ? | - |
? | |
morphine + UDP-glucuronate | - |
Rattus norvegicus | UDP + morphine 3,6-diglucuronide | - |
? | |
morphine + UDP-glucuronate | - |
Rattus norvegicus Wistar | UDP + morphine 3,6-diglucuronide | - |
? | |
N-hydroxy-2-naphthylamine + UDPglucuronate | - |
Rattus norvegicus | UDP + N-hydroxy-2-naphthylamine-O-glucuronide | - |
? | |
N-hydroxy-2-naphthylamine + UDPglucuronate | - |
Rattus norvegicus Wistar | UDP + N-hydroxy-2-naphthylamine-O-glucuronide | - |
? | |
testosterone + UDPglucuronate | - |
Rattus norvegicus | UDP + testosterone O-glucuronide | - |
? | |
UDP-alpha-D-glucuronate + chloramphenicol | - |
Rattus norvegicus | UDP + chloramphenicol beta-D-glucuronide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, phenobarbital-inducible isozyme | Rattus norvegicus |
? | additional values for rat isozymes ranging from 51000 to 59000 | Rattus norvegicus |
? | x * 54000, methylcholanthrene-inducible isozyme | Rattus norvegicus |
? | all values determined by SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-20 | - |
rapid loss of activity | Rattus norvegicus |