Literature summary for 2.4.1.162 extracted from

Gabrielczyk, J.; Kluitmann, J.; Dammeyer, T.; Joerdening, H.J.
Effects of ionic strength on inclusion body refolding at high concentration (2017), Protein Expr. Purif., 130, 100-106 .

Search for more literature for 2.4.1.162

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of C-terminally Strep-tagged enzyme in Escherichia coli strain BL21(DE3) Rosetta in inclusion bodies	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-
Bacillus subtilis NCIMB 11871	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble C-terminally Strep-tagged enzyme from Escherichia coli strain BL21 (DE3) Rosetta inclusion bodies by Strep-Tactin affinity chromatography and dialysis	Bacillus subtilis

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant C-terminally Strep-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta inclusion bodies, method optimization and evaluation of a cost effective renaturation, detailed overview. The enzyme is solubilized from batch sample by detergent buffer containing 1 M urea, 0.1 M Tris, 25 mM deoxycholate, and 1% v/v IGEPAL CA-630. Refolding is done by batchwise or continuous exchange of dialysis buffer with cellulose membrane, over a period of 48 h and a buffer to sample volume ratio of 100:1 at 4°C, batch dialysis. Mild solubilization at low urea concentration (2 M) and high pH (pH 12) does not improve the recovery of protein from inclusion bodies. Phosphate buffers exhibit above-average activity yield of 1022 U/ml	Bacillus subtilis

Renatured (Comment)

Organism

recombinant C-terminally Strep-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta inclusion bodies, method optimization and evaluation of a cost effective renaturation, detailed overview. The enzyme is solubilized from batch sample by detergent buffer containing 1 M urea, 0.1 M Tris, 25 mM deoxycholate, and 1% v/v IGEPAL CA-630. Refolding is done by batchwise or continuous exchange of dialysis buffer with cellulose membrane, over a period of 48 h and a buffer to sample volume ratio of 100:1 at 4°C, batch dialysis. Mild solubilization at low urea concentration (2 M) and high pH (pH 12) does not improve the recovery of protein from inclusion bodies. Phosphate buffers exhibit above-average activity yield of 1022 U/ml

Bacillus subtilis

Subunits

Subunits	Comment	Organism
monomer	1 * 51620, sequence calculation and SDS-PAGE	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
fructosyltransferase	-	Bacillus subtilis
FTF	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bacillus subtilis