Literature summary for 2.4.1.16 extracted from

Salgado-Lugo, H.; Sanchez-Arreguin, A.; Ruiz-Herrera, J.
Heterologous expression of an active chitin synthase from Rhizopus oryzae (2016), Fungal Genet. Biol., 97, 10-17 .

Search for more literature for 2.4.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
N-acetyl-D-glucosamine	1.28fold stimulation of activity at 20 mM	Rhizopus arrhizus

Cloned(Commentary)

Cloned (Comment)	Organism
N-terminally deleted enzyme is expressed in Escherichia coli BL21 Star (DE3) cells	Rhizopus arrhizus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
32.1	-	UDP-N-acetyl-D-glucosamine	at pH 6.5 and 25°C	Rhizopus arrhizus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	may replace the activating effect of Mg2+	Rhizopus arrhizus
Co2+	may replace the activating effect of Mg2+	Rhizopus arrhizus
Mg2+	5 mM used in assay conditions	Rhizopus arrhizus
Mn2+	may replace the activating effect of Mg2+	Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n	Rhizopus arrhizus	-	UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhizopus arrhizus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography	Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n	-	Rhizopus arrhizus	UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1	-	?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosamine	-	Rhizopus arrhizus	UDP + 1,4-(N-acetyl-beta-D-glucosaminyl)2	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, recombinant enzyme, SDS-PAGE	Rhizopus arrhizus
?	x * 57000, native enzyme, SDS-PAGE	Rhizopus arrhizus

Synonyms

Synonyms	Comment	Organism
Chs1	-	Rhizopus arrhizus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	40	the enzyme is active between 15 and 40°C	Rhizopus arrhizus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	the enzyme is active between pH 5.0 and 8.0	Rhizopus arrhizus

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Release 2023.1 (January 2023)