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Literature summary for 2.4.1.16 extracted from

  • Bahmed, K.; Quiles, F.; Wathier, M.; Bonaly, R.; Benallaoua, S.; Pucci, B.; Coulon, J.
    Use of dansyl N-acetyl glucosamine as substrate for chitin synthetase activities (2005), Proc. Biochem., 40, 2523-2529.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Nikkomycin 0.015 for chitin synthetase III; IC50: 0.022 mM for chitin synthetase II; IC50: 0.032 mM for chitin synthetase I Kluyveromyces bulgaricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0133
-
6-O-dansyl-N-acetylglucosamine chitin synthetase I Kluyveromyces bulgaricus
0.2
-
6-O-dansyl-N-acetylglucosamine chitin synthetase III Kluyveromyces bulgaricus
0.5
-
6-O-dansyl N-acetylglucosamine chitin synthetase II Kluyveromyces bulgaricus

Organism

Organism UniProt Comment Textmining
Kluyveromyces bulgaricus
-
ATCC 96631
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0025
-
chitin synthetase III, activity at the end of exponential growth phase Kluyveromyces bulgaricus
0.0028
-
chitin synthetase II, activity at the end of exponential growth phase Kluyveromyces bulgaricus
0.008
-
chitin synthetase I, activity at the end of exponential growth phase Kluyveromyces bulgaricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-dansyl-N-acetylglucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
Kluyveromyces bulgaricus ?
-
?
acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
Kluyveromyces bulgaricus UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
?

Synonyms

Synonyms Comment Organism
chitin synthetase I
-
Kluyveromyces bulgaricus
chitin synthetase II
-
Kluyveromyces bulgaricus
chitin synthetase III
-
Kluyveromyces bulgaricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.022
-
IC50: 0.022 mM for chitin synthetase II Kluyveromyces bulgaricus Nikkomycin
0.032
-
IC50: 0.032 mM for chitin synthetase I Kluyveromyces bulgaricus Nikkomycin