Literature summary for 2.4.1.16 extracted from

Causier, B.E.; Milling, R.J.; Foster, S.G.; Adams, D.J.
Characterization of chitin synthase from Botrytis cinerea (1994), Microbiology, 140, 2199-2205.

No PubMed abstract available

Search for more literature for 2.4.1.16

Activating Compound

Activating Compound	Comment	Organism	Structure
GlcNAc	stimulates	Botrytis cinerea

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Botrytis cinerea

Inhibitors

Inhibitors	Comment	Organism	Structure
edifenphos	non-competitive	Botrytis cinerea
nikkomycin Z	-	Botrytis cinerea
Polyoxin D	-	Botrytis cinerea
UDP	-	Botrytis cinerea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	UDP-GlcNAc	-	Botrytis cinerea

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	dependent upon presence of divalent cation, Mn2+ is most effective	Botrytis cinerea

Organism

Organism	UniProt	Comment	Textmining
Botrytis cinerea	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	preincubation with trypsin results in approximately 4fold increase in activity	Botrytis cinerea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n	-	Botrytis cinerea	UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Botrytis cinerea

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0001	-	nikkomycin Z	-	Botrytis cinerea
0.006	-	Polyoxin D	-	Botrytis cinerea
0.054	-	edifenphos	-	Botrytis cinerea
0.19	-	UDP	-	Botrytis cinerea

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Release 2023.1 (January 2023)