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Literature summary for 2.4.1.16 extracted from

  • Cabib, E.; Kang, M.S.; Au-Young, J.
    Chitin synthase from Saccharomyces cerevisiae (1987), Methods Enzymol., 138, 643-649.
    View publication on PubMed
Search for more literature for 2.4.1.16

Activating Compound

Activating Compound Comment Organism Structure
GlcNAc stimulates Saccharomyces cerevisiae
GlcNAc Km: 4.7 mM Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
chitin oligosaccharides slight Saccharomyces cerevisiae
Nikkomycin
-
 Saccharomyces cerevisiae
Polyoxin D
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 UDP-GlcNAc
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane integral membrane protein Saccharomyces cerevisiae 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for a divalent cation. Mg2+, Mn2+ or Co2+ Saccharomyces cerevisiae
Mg2+ absolute requirement for a divalent cation. Mg2+, Mn2+ or Co2+. Optimum concentration for Mg2+ is 1-10 mM Saccharomyces cerevisiae
Mn2+ absolute requirement for a divalent cation. Mg2+, Mn2+ or Co2+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme is present in the cell in a zymogen form Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.7
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, digitonin-solubilized enzyme is stable for several months, purified enzyme is stable for at least a few weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Saccharomyces cerevisiae UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 trypsin-activated enzyme Saccharomyces cerevisiae