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Literature summary for 2.4.1.155 extracted from
- Characterization of UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,6-N-Acetylglucosaminyltransferase V from a Human Hepatoma Cell Line Hep3B (1999), Arch. Biochem. Biophys., 367, 281-288.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A592G | increased specific activity | Homo sapiens |
| T555R | increased specific activity | Homo sapiens |
| V375L | increased specific activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-N-acetylglucosamine | 20 mM | Homo sapiens |  |
| CDP-N-acetylglucosamine | 20 mM | Homo sapiens | |
| Fe3+ | 1 mM inhibits | Homo sapiens | |
| GDP-N-acetylglucosamine | 20 mM | Homo sapiens | |
| N-bromosuccinimide | 1 mM inhibits | Homo sapiens | |
| TDP-N-acetylglucosamine | 20 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.8 | - |
UDP-N-acetylglucosamine | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM stimulates | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
native PAGE | Homo sapiens |
| 67000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colonic cancer cell line | - |
Homo sapiens | - |
| HEP-3B cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 110 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | most active towards biantennary sugars | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | stable for 24 h at 4°C | Homo sapiens |
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Release 2023.1 (January 2023)
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