Literature summary for 2.4.1.155 extracted from

Shoreibah, M.; Hindsgaul, O.; Pierce, M.
Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase (1992), J. Biol. Chem., 267, 2920-2927.

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Activating Compound

Activating Compound	Comment	Organism	Structure
glycerol	activation and stabilization, 20%	Rattus norvegicus
Immunoglobulin G	activation and stabilization, 0.5 mg/ml	Rattus norvegicus
NaCl	stabilizes and enhances activity, 0.2 M	Rattus norvegicus
Triton X-100	activation, 1.0-1.5%	Rattus norvegicus

General Stability

General Stability	Organism
glycerol, 20%, stabilizes and enhances activity	Rattus norvegicus
immunoglobulin G, 0.5 mg/ml, stabilizes and enhances activity	Rattus norvegicus
NaCl, 0.2 M, stabilizes and enhances activity	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibitor: EDTA	Rattus norvegicus
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)8-COOCH3	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.087	-	N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)8COOCH3	-	Rattus norvegicus
11	-	UDP-N-acetylglucosamine	-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Rattus norvegicus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no requirement for Mn2+	Rattus norvegicus
additional information	no requirement for Mn2+, Mg2+, Ca2+	Rattus norvegicus
NaCl	activation, 0.2 M	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
69000	-	SDS-PAGE, 2 bands: 69000 and 75000	Rattus norvegicus
75000	-	SDS-PAGE, 2 bands: 69000 and 75000	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.22	-	-	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4°C, several months in 20% glycerol	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	-	Rattus norvegicus	UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R	-	?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)8COOCH3	-	Rattus norvegicus	UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-O-(CH2)8COOCH3	-	?

Subunits

Subunits	Comment	Organism
More	rat kidney enzyme appears as doublet of MW 69000 and 75000 on SDS-PAGE	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.141	-	N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)8-COOCH3	-	Rattus norvegicus

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Release 2023.1 (January 2023)