Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.150 extracted from

  • Mattila, P.; Salminen, H.; Hirvas, L.; Niittymäki, J.; Salo, H.; Niemelä, R.; Fukuda, M.; Renkonen, O.; Renkonen, R.
    The centrally acting beta1,6N-acetylglucosaminyltransferase (GlcNAc to Gal). Functional expression, purification, and acceptor specificity of a human enzyme involved in midchain branching of linear poly-N-acetyllactosamines (1998), J. Biol. Chem., 273, 27633-27639.
    View publication on PubMed
Search for more literature for 2.4.1.150

Application

Application Comment Organism
synthesis cIGnT6 transfers multiple GlcNAc branches to long linear polylactosamines, a prerequisite for improving enzyme-assisted in vitro synthesis of a type of multivalent sialyl Lewis x glycans that are high affinity inhibitors of lymphocyte L-selectin, enzyme allows general polylactosamine synthesis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
IGnT6 is cloned, sequenced and cDNA encoding cIGnT6 in PA-1 cells is expressed in SF9 insect cells as protein fused to glutathione S-transferase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Homo sapiens responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant enzyme, expressed in SF9 insect cells as fusion protein with glutathione S-transferase has 5 potential N-glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme, expressed in SF9 insect cells as protein fused to glutathione S-transferase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
PA-1 cell cIGnT6, embryonal carcinoma cell line Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, recombinant enzyme, a few days, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues Homo sapiens ?
-
 ?
additional information no substrate of cIGnT6: GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)(Fucalpha(1-3))GlcNAc, no transfer of GlcNAc to substrates with alpha1-3-fucosyl residues and to midchain galactoses that belongs to Lewis x determinants Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT Homo sapiens UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT Homo sapiens UDP + (GlcNAc)1-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc also as product: (GlcNAc)2-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, cIGnT6 generates in a partial reaction nona- and decasaccharide products, which represent mixtures of isomers carrying one or two GlcNAc-branches on the linear octasaccharide acceptor ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine forms branches in poly-N-acetyllactosamines Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine cIGnT6 transfers one or multiple GlcNAc branches to midchain galactoses of long linear polylactosamines Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67000-74000, glycosylated protein fused to glutathione S-transferase, size heterogenicity is at least partially due to differences in N-glycosylation, SDS-PAGE Homo sapiens