Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.15 extracted from

  • Zaparty, M.; Hagemann, A.; Bräsen, C.; Hensel, R.; Lupas, A.N.; Brinkmann, H.; Siebers, B.
    The first prokaryotic trehalose synthase complex identified in the hyperthermophilic crenarchaeon Thermoproteus tenax. (2013), PLoS One, 8, e61354.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
glycosyltransferase the Thermoproteus tenax trehalose-6-phosphate synthase/phosphatase (TPSP) exhibits high phosphatase activity, but requires activation by the co-expressed glycosyltransferase for bifunctional synthase/phosphatase activity. The glycosyltransferase mediated activation of trehalose-6-phosphate synthase activity relies on the fusion of both, trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase domain, in the TPSP enzyme. Activation is mediated by complex-formation in vivo Thermoproteus tenax

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 81000, TPSP holoenzyme, x * 50000, isolated trehalose-6-phosphate synthase domain, x * 33000, isolated trehalose-6-phosphate phophatase domain Thermoproteus tenax
50000
-
x * 81000, TPSP holoenzyme, x * 50000, isolated trehalose-6-phosphate synthase domain, x * 33000, isolated trehalose-6-phosphate phophatase domain Thermoproteus tenax
81000
-
x * 81000, TPSP holoenzyme, x * 50000, isolated trehalose-6-phosphate synthase domain, x * 33000, isolated trehalose-6-phosphate phophatase domain Thermoproteus tenax

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax G4RK44
-
-
Thermoproteus tenax G4RK44 bifunctional trehalose-6-phosphate synthase/phosphatase, EC 2.4.1.15 and EC 3.1.3.12
-
Thermoproteus tenax ATCC 35583 G4RK44 bifunctional trehalose-6-phosphate synthase/phosphatase, EC 2.4.1.15 and EC 3.1.3.12
-
Thermoproteus tenax DSM 2078 G4RK44
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
pH 7.0, 80°C Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose 6-phosphate + H2O
-
Thermoproteus tenax alpha,alpha-trehalose + phosphate
-
?
alpha,alpha-trehalose 6-phosphate + H2O
-
Thermoproteus tenax DSM 2078 alpha,alpha-trehalose + phosphate
-
?
alpha,alpha-trehalose 6-phosphate + H2O
-
Thermoproteus tenax ATCC 35583 alpha,alpha-trehalose + phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Thermoproteus tenax UDP + alpha,alpha-1,1-trehalose 6-phosphate presence of glycosyltransferase protein is required ?
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Thermoproteus tenax DSM 2078 UDP + alpha,alpha-1,1-trehalose 6-phosphate presence of glycosyltransferase protein is required ?
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Thermoproteus tenax ATCC 35583 UDP + alpha,alpha-1,1-trehalose 6-phosphate presence of glycosyltransferase protein is required ?

Subunits

Subunits Comment Organism
? x * 81000, TPSP holoenzyme, x * 50000, isolated trehalose-6-phosphate synthase domain, x * 33000, isolated trehalose-6-phosphate phophatase domain Thermoproteus tenax

Synonyms

Synonyms Comment Organism
TPSP
-
Thermoproteus tenax
TPSP the bifunctional enzyme consists of an N-terminal trehalose-6-phosphate synthase and a C-terminal trehalose-6-phosphate phosphatase domain Thermoproteus tenax
trehalose-6-phosphate synthase/phosphatase the bifunctional enzyme consists of an N-terminal trehalose-6-phosphate synthase and a C-terminal trehalose-6-phosphate phosphatase domain Thermoproteus tenax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Thermoproteus tenax

General Information

General Information Comment Organism
physiological function the fused trehalose-6-phosphate synthase/phosphatase TPSP consists of an N-terminal trehalose-6-phosphate synthase (TPS) and a C-terminal trehalose-6-phosphate phosphatase (TPP) domain. The gene is organized in an operon with a putative glycosyltransferase GT and a putative mechanosensitive channel MSC. The enzyme exhibits high phosphatase activity, but requires activation by the co-expressed GT for bifunctional synthase-phosphatase activity. The GT mediated activation of trehalose-6-phosphate synthase activity relies on the fusion of both, trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase domain, in the enzyme. Activation is mediated by complex-formation Thermoproteus tenax