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Literature summary for 2.4.1.15 extracted from

  • Sengupta, S.; Lahiri, S.; Banerjee, S.; Bashistha, B.; Ghosh, A.K.
    Arginine mediated purification of trehalose-6-phosphate synthase (TPS) from Candida utilis: its characterization and regulation (2011), Biochim. Biophys. Acta, 1810, 1346-1354.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CDTA
-
Cyberlindnera jadinii
chondroitin sulfate polyanions heparin and chondroitin sulfate stimulate TPS activity with different glucosyl donors Cyberlindnera jadinii
EGTA
-
Cyberlindnera jadinii
heparin polyanions heparin and chondroitin sulfate stimulate TPS activity with different glucosyl donors Cyberlindnera jadinii
iodoacetamide activates 39% at 1 mM Cyberlindnera jadinii
iodoacetic acid activates 47.5% at 1 mM Cyberlindnera jadinii
additional information dependence on polyanion concentrations for optimum TPS activity, overview Cyberlindnera jadinii
RNA
-
Cyberlindnera jadinii

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 45% inhibition at 5 mM Cyberlindnera jadinii
Cd2+ slightly inhibitory Cyberlindnera jadinii
Fe2+ slightly inhibitory Cyberlindnera jadinii
N-ethylmaleimide 68% inhibition at 1 mM Cyberlindnera jadinii

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates 33% at 5 mM Cyberlindnera jadinii
Mg2+ 2.5fold activation at 5 mM, best at 70 mM Cyberlindnera jadinii
Mn2+ best divalent cation, 2.6fold activation at 5 mM, best at 70 mM Cyberlindnera jadinii
additional information no or poor effects by CoCl2, CaCl2, BaCl2, and FeSO4 Cyberlindnera jadinii
Zn2+ 2.2fold activation at 5 mM Cyberlindnera jadinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate Cyberlindnera jadinii
-
UDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate Cyberlindnera jadinii NCIM/ Y500
-
UDP + alpha,alpha-trehalose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Cyberlindnera jadinii
-
-
-
Cyberlindnera jadinii NCIM/ Y500
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 22.1fold to homogeneity by several gel filtration steps Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
TPS activity is maximally at pH 8.5 with 2.11 U/mg, followed by 2.01 U/mg at pH 9.0, 1.93 U/mg at pH 9.5, and 1.21 U/mg at pH 10.5, but only 0.31 U/mg at pH 4.5 Cyberlindnera jadinii
0.096
-
crude enzyme, pH 8.5, 37°C Cyberlindnera jadinii
2.03
-
purified native enzyme, pH 8.5, 37°C Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-glucose is the best donor substrate Cyberlindnera jadinii ADP + alpha,alpha-trehalose 6-phosphate
-
?
ADP-alpha-D-glucose + D-glucose 6-phosphate ADP-glucose is the best donor substrate Cyberlindnera jadinii NCIM/ Y500 ADP + alpha,alpha-trehalose 6-phosphate
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate cf. EC 2.4.1.36 Cyberlindnera jadinii GDP + alpha,alpha-trehalose 6-phosphate
-
?
GDP-alpha-D-glucose + D-glucose 6-phosphate cf. EC 2.4.1.36 Cyberlindnera jadinii NCIM/ Y500 GDP + alpha,alpha-trehalose 6-phosphate
-
?
additional information ADP-gucose is the best donor substrate, and displays highest activity of 4.23 U/mg, followed by UDPG with 2.016 U/mg and GDPG with 1.609 U/mg. TDPG displays activity of 0.95 U/mg Cyberlindnera jadinii ?
-
?
additional information ADP-gucose is the best donor substrate, and displays highest activity of 4.23 U/mg, followed by UDPG with 2.016 U/mg and GDPG with 1.609 U/mg. TDPG displays activity of 0.95 U/mg Cyberlindnera jadinii NCIM/ Y500 ?
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
-
Cyberlindnera jadinii TDP + alpha,alpha-trehalose 6-phosphate
-
?
TDP-alpha-D-glucose + D-glucose 6-phosphate
-
Cyberlindnera jadinii NCIM/ Y500 TDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
Cyberlindnera jadinii UDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate
-
Cyberlindnera jadinii NCIM/ Y500 UDP + alpha,alpha-trehalose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Cyberlindnera jadinii

Synonyms

Synonyms Comment Organism
TPS
-
Cyberlindnera jadinii
trehalose-6-phosphate synthase
-
Cyberlindnera jadinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Cyberlindnera jadinii

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10.5 TPS activity is maximally at pH 8.5 with 2.11 U/mg, followed by 2.01 U/mg at pH 9.0, 1.93 U/mg at pH 9.5, and 1.21 U/mg at pH 10.5, but only 0.31 U/mg at pH 4.5 Cyberlindnera jadinii

General Information

General Information Comment Organism
additional information trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase form a complex Cyberlindnera jadinii