Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CDTA | - |
Cyberlindnera jadinii | |
chondroitin sulfate | polyanions heparin and chondroitin sulfate stimulate TPS activity with different glucosyl donors | Cyberlindnera jadinii | |
EGTA | - |
Cyberlindnera jadinii | |
heparin | polyanions heparin and chondroitin sulfate stimulate TPS activity with different glucosyl donors | Cyberlindnera jadinii | |
iodoacetamide | activates 39% at 1 mM | Cyberlindnera jadinii | |
iodoacetic acid | activates 47.5% at 1 mM | Cyberlindnera jadinii | |
additional information | dependence on polyanion concentrations for optimum TPS activity, overview | Cyberlindnera jadinii | |
RNA | - |
Cyberlindnera jadinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 45% inhibition at 5 mM | Cyberlindnera jadinii | |
Cd2+ | slightly inhibitory | Cyberlindnera jadinii | |
Fe2+ | slightly inhibitory | Cyberlindnera jadinii | |
N-ethylmaleimide | 68% inhibition at 1 mM | Cyberlindnera jadinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates 33% at 5 mM | Cyberlindnera jadinii | |
Mg2+ | 2.5fold activation at 5 mM, best at 70 mM | Cyberlindnera jadinii | |
Mn2+ | best divalent cation, 2.6fold activation at 5 mM, best at 70 mM | Cyberlindnera jadinii | |
additional information | no or poor effects by CoCl2, CaCl2, BaCl2, and FeSO4 | Cyberlindnera jadinii | |
Zn2+ | 2.2fold activation at 5 mM | Cyberlindnera jadinii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Cyberlindnera jadinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Cyberlindnera jadinii | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | Cyberlindnera jadinii NCIM/ Y500 | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Cyberlindnera jadinii NCIM/ Y500 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 22.1fold to homogeneity by several gel filtration steps | Cyberlindnera jadinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
TPS activity is maximally at pH 8.5 with 2.11 U/mg, followed by 2.01 U/mg at pH 9.0, 1.93 U/mg at pH 9.5, and 1.21 U/mg at pH 10.5, but only 0.31 U/mg at pH 4.5 | Cyberlindnera jadinii |
0.096 | - |
crude enzyme, pH 8.5, 37°C | Cyberlindnera jadinii |
2.03 | - |
purified native enzyme, pH 8.5, 37°C | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose 6-phosphate | ADP-glucose is the best donor substrate | Cyberlindnera jadinii | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
ADP-alpha-D-glucose + D-glucose 6-phosphate | ADP-glucose is the best donor substrate | Cyberlindnera jadinii NCIM/ Y500 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | cf. EC 2.4.1.36 | Cyberlindnera jadinii | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | cf. EC 2.4.1.36 | Cyberlindnera jadinii NCIM/ Y500 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
additional information | ADP-gucose is the best donor substrate, and displays highest activity of 4.23 U/mg, followed by UDPG with 2.016 U/mg and GDPG with 1.609 U/mg. TDPG displays activity of 0.95 U/mg | Cyberlindnera jadinii | ? | - |
? | |
additional information | ADP-gucose is the best donor substrate, and displays highest activity of 4.23 U/mg, followed by UDPG with 2.016 U/mg and GDPG with 1.609 U/mg. TDPG displays activity of 0.95 U/mg | Cyberlindnera jadinii NCIM/ Y500 | ? | - |
? | |
TDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Cyberlindnera jadinii | TDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
TDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Cyberlindnera jadinii NCIM/ Y500 | TDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Cyberlindnera jadinii | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Cyberlindnera jadinii NCIM/ Y500 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Cyberlindnera jadinii |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Cyberlindnera jadinii |
trehalose-6-phosphate synthase | - |
Cyberlindnera jadinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cyberlindnera jadinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Cyberlindnera jadinii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | TPS activity is maximally at pH 8.5 with 2.11 U/mg, followed by 2.01 U/mg at pH 9.0, 1.93 U/mg at pH 9.5, and 1.21 U/mg at pH 10.5, but only 0.31 U/mg at pH 4.5 | Cyberlindnera jadinii |
General Information | Comment | Organism |
---|---|---|
additional information | trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase form a complex | Cyberlindnera jadinii |