Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
estimated by HPGPLC using a Ultropak G2000SW column and by SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Saccharomyces cerevisiae | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
crude extract is prepared, the enzyme is isolated by ammonium sulfate fractionation and high performance gel permeation liquid chromatography, using a HiLoad 16/60 Superdex 200 and a Ultropak TSK G2000SW column | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the activity of trehalose-6-phosphate synthase is concentration dependent | Saccharomyces cerevisiae |
0.01 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step soluble supernatant | Saccharomyces cerevisiae |
0.49 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step ammonimum sulfate fractionation | Saccharomyces cerevisiae |
0.74 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step HPGPLC on TSK G2000SW | Saccharomyces cerevisiae |
1.58 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step HPGPLC on HiLoad 16/60 Superdex 200 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Saccharomyces cerevisiae | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha,alpha-trehalose-phosphate synthase (UDP-forming) | - |
Saccharomyces cerevisiae |
TPS | - |
Saccharomyces cerevisiae |
trehalose-6-phosphate synthase | key subunit of the trehalose synthase complex | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity assay | Saccharomyces cerevisiae |