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Literature summary for 2.4.1.149 extracted from

  • Seko, A.; Yamashita, K.
    beta1,3-N-Acetylglucosaminyltransferase-7 (beta3Gn-T7) acts efficiently on keratan sulfate-related glycans (2004), FEBS Lett., 556, 216-220.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells, localization in membranes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA slightly, 12% inhibition at 10 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc recombinant enzyme, pH 7.2, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about one third of the activity compared to Mn2+, recombinant enzyme Homo sapiens
Mg2+ about one third of the activity compared to Mn2+, recombinant enzyme Homo sapiens
Mn2+ required for activity, recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line Homo sapiens ?
-
?
additional information substrate specificity, enzyme acts efficiently on keratan sulfate-related glycans, while lacto-N-tetraose and lacto-N-neo-tetraose are poor substrates, overview, product analysis Homo sapiens ?
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc i.e. L2L4, 72% activity compared to L2L2 Homo sapiens UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate Homo sapiens UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate Homo sapiens UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc
-
?

Synonyms

Synonyms Comment Organism
beta1,3-N-acetylglucosaminyltransferase-7
-
Homo sapiens
beta3Gn-T7
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Homo sapiens