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Literature summary for 2.4.1.149 extracted from
- Purification and characterization of UDP-GlcNAc:Galbeta 1-4Glc(NAc)beta-1,3-N-acetylglucosaminyltransferase (poly-N-acetyllactosamine extension enzyme) from calf serum (1993), J. Biol. Chem., 268, 27118-27126.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bos taurus |  |
| 4-Thiouridine diphosphate | inhibits in a concentration-dependent manner | Bos taurus | |
| CH3CN | - |
Bos taurus | |
| N-acetyllactosamine | strong product inhibition | Bos taurus | |
| SDS | - |
Bos taurus | |
| UDP | 1 mM, 70% inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.129 | - |
UDP-N-acetyl-D-glucosamine | - |
Bos taurus | |
| 18.2 | - |
lactose | - |
Bos taurus | |
| 19.6 | - |
N-acetyllactosamine | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slightly stimulates, 12.8% as effective as Mn2+ at 10 mM | Bos taurus | |
| Mn2+ | strict requirement | Bos taurus | |
| Mn2+ | optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+ | Bos taurus | |
| additional information | enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+ | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
1 * 70000, SDS-PAGE | Bos taurus |
| 90000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + glycolipid | Bos taurus | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + keratan sulfate | Bos taurus | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-glycan | Bos taurus | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + O-glycan | Bos taurus | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | Bos taurus | involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
newborn calf | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 125000fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | newborn calf serum | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.118 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, at least 2 weeks, stable | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc | i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine | Bos taurus | UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
| UDP-N-acetyl-D-glucosamine + glycolipid | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + keratan sulfate | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-glycan | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + O-glycan | physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme | Bos taurus | ? | - |
? | |
| UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein | best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc | Bos taurus | UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein | - |
? | |
| UDP-N-acetylglucosamine + lactose | transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine | Bos taurus | UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
| UDP-N-acetylglucosamine + N-acetyllactosamine | transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage | Bos taurus | UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
| 42 | - |
112% of activity compared with that at 37°C | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
4% of activity compared with that at 37°C | Bos taurus |
| 12 | - |
9% of activity compared with that at 37°C | Bos taurus |
| 20 | - |
28% of activity compared with that at 37°C | Bos taurus |
| 30 | - |
63% of activity compared with that at 37°C | Bos taurus |
| 42 | - |
112% of activity compared with that at 37°C | Bos taurus |
| 45 | - |
88% of activity compared with that at 37°C | Bos taurus |
| 50 | - |
10% of activity compared with that at 37°C | Bos taurus |
| 55 | - |
2% of activity compared with that at 37°C | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
broad pH-optimum around pH 7 | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | substantial reaction in the range, 65% of activity at pH 7 | Bos taurus |
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