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Literature summary for 2.4.1.148 extracted from

  • Pak, J.E.; Satkunarajah, M.; Seetharaman, J.; Rini, J.M.
    Structural and mechanistic characterization of leukocyte-type core 2 ?1,6-N-acetylglucosaminyltransferase: a metal-ion-independent GT-A glycosyltransferase (2011), J. Mol. Biol., 414, 798-811.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme C217S is expressed in HEK-293T cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme C217S in complex with UDP, hanging drop vapor diffusion method, using 24% (w/v) polyethylene glycol 4000, 0.1 M glycine (pH 9.0) and 0.6 M LiCl Mus musculus

Protein Variants

Protein Variants Comment Organism
C217S the mutant is not oxidatively inactivated, and its kinetic parameters are very similar to those of the native enzyme Mus musculus
K401A/C217S catalytically inactive Mus musculus
R378A/C217S catalytically inactive Mus musculus
R378A/K401A/C217S catalytically inactive Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
UDP
-
Mus musculus
UMP
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
UDP-N-acetyl-D-glucosamine mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal-ion-independent enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr Mus musculus
-
UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG-Sepahrose beads chromatography, gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
Mus musculus UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosaminyl-4-nitrophenyl
-
Mus musculus UDP + N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,3)-N-acetyl-D-galactosaminyl-4-nitrophenyl
-
?

Synonyms

Synonyms Comment Organism
C2GnT-I
-
Mus musculus
C2GnT-L
-
Mus musculus
leukocyte-type core 2 beta1,6-N-acetylglucosaminyltransferase
-
Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.23
-
UMP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus
0.26
-
UDP mutant enzyme C217S, in 50 mM Tris (pH 7.5), at 37°C Mus musculus