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Literature summary for 2.4.1.147 extracted from

  • Saito, F.; Sakamoto, I.; Kanatani, A.; Chiba, Y.
    Manganese ion concentration affects production of human core 3 O-glycan in Saccharomyces cerevisiae (2016), Biochim. Biophys. Acta, 1860, 1809-1820 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene B3GNT6, recombinant expression in Saccharomyces cerevisiae, the human beta3Gn-T6 is produced as a protein fused with the transmembrane domains of Saccharomyces cerevisiae glycosyltransferases (ScMnn9p, ScMnn2p, ScMnn1p, or ScGnt1p), thereby localizing beta3Gn-T6 to the Golgi, optimization of beta3Gn-T6 expression Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information introduction of a codon-optimized human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (beta3Gn-T6) gene into Saccharomyces cerevisiae yields increases in beta3Gn-T6 activity but does not alter the level of core 3 production. The highest in vitro activity of beta3Gn-T6 is observed at Mn2+ concentrations of 10 mM and above. Supplementation of MnCl2 to the culture medium yields increases of up to 25% in the accumulation of core 3 on the MUC1ap. The yeast invertase from the core 3-producing strain is less extensively N-glycosylated, but it is partially restored by the addition of MnCl2 to the medium. Physiological Mn2+ concentration in Saccharomyces cerevisiae is insufficient to facilitate optimal synthesis of core 3. Mn2+ supplementation leads to upregulation of reaction of glycosylation in the Golgi, resulting in increases of core 3 production. Control of Mn2+ concentration is important for production of specific mammalian-type glycans in Sacchromyces cerevisiae microsomes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Homo sapiens 5794
-
membrane
-
Homo sapiens 16020
-
microsome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on, physiological Mn2+ concentration in Saccharomyces cerevisiae is insufficient to facilitate optimal synthesis of core 3. Mn2+ supplementation led to upregulation of reaction of glycosylation in the Golgi, resulting in increases of core 3 production. Although the supply of Mn2+ in the Golgi apparatus is essential for glycosylation, excess Mn2+ levels are toxic to yeast Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] Homo sapiens
-
UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6ZMB0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
Homo sapiens
-
small intestine
-
Homo sapiens
-
stomach
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-GlcNAc + 4-nitrophenyl-alpha-N-acetylgalactosamine
-
Homo sapiens UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosamine
-
?
UDP-GlcNAc + GlcNAc
-
Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine
-
?
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
-
Homo sapiens UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
-
?

Synonyms

Synonyms Comment Organism
B3GNT6
-
Homo sapiens
beta3Gn-T6
-
Homo sapiens
core 3 synthase
-
Homo sapiens
UDPGlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
-
Homo sapiens
uridine diphosphate
-
Homo sapiens
[UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function in humans, the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-O-Ser/Thr) has been found in mucins from specific tissues such as the stomach, small intestine, and colon, and the human gene (B3GNT6) encoding the core 3 synthase (uridine diphosphate [UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase, beta3Gn-T6) is involved in core 3 synthesis Homo sapiens