Cloned (Comment) | Organism |
---|---|
gene B3GNT6, recombinant expression in Saccharomyces cerevisiae, the human beta3Gn-T6 is produced as a protein fused with the transmembrane domains of Saccharomyces cerevisiae glycosyltransferases (ScMnn9p, ScMnn2p, ScMnn1p, or ScGnt1p), thereby localizing beta3Gn-T6 to the Golgi, optimization of beta3Gn-T6 expression | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of a codon-optimized human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (beta3Gn-T6) gene into Saccharomyces cerevisiae yields increases in beta3Gn-T6 activity but does not alter the level of core 3 production. The highest in vitro activity of beta3Gn-T6 is observed at Mn2+ concentrations of 10 mM and above. Supplementation of MnCl2 to the culture medium yields increases of up to 25% in the accumulation of core 3 on the MUC1ap. The yeast invertase from the core 3-producing strain is less extensively N-glycosylated, but it is partially restored by the addition of MnCl2 to the medium. Physiological Mn2+ concentration in Saccharomyces cerevisiae is insufficient to facilitate optimal synthesis of core 3. Mn2+ supplementation leads to upregulation of reaction of glycosylation in the Golgi, resulting in increases of core 3 production. Control of Mn2+ concentration is important for production of specific mammalian-type glycans in Sacchromyces cerevisiae microsomes | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | dependent on, physiological Mn2+ concentration in Saccharomyces cerevisiae is insufficient to facilitate optimal synthesis of core 3. Mn2+ supplementation led to upregulation of reaction of glycosylation in the Golgi, resulting in increases of core 3 production. Although the supply of Mn2+ in the Golgi apparatus is essential for glycosylation, excess Mn2+ levels are toxic to yeast | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] | Homo sapiens | - |
UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6ZMB0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Homo sapiens | - |
small intestine | - |
Homo sapiens | - |
stomach | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GlcNAc + 4-nitrophenyl-alpha-N-acetylgalactosamine | - |
Homo sapiens | UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosamine | - |
? | |
UDP-GlcNAc + GlcNAc | - |
Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] | - |
Homo sapiens | UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
B3GNT6 | - |
Homo sapiens |
beta3Gn-T6 | - |
Homo sapiens |
core 3 synthase | - |
Homo sapiens |
UDPGlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 | - |
Homo sapiens |
uridine diphosphate | - |
Homo sapiens |
[UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | in humans, the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-O-Ser/Thr) has been found in mucins from specific tissues such as the stomach, small intestine, and colon, and the human gene (B3GNT6) encoding the core 3 synthase (uridine diphosphate [UDP]-GlcNAc:betaGal beta1,3-N-acetylglucosaminyltransferase, beta3Gn-T6) is involved in core 3 synthesis | Homo sapiens |