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Literature summary for 2.4.1.145 extracted from

  • Oguri, S.; Yoshida, A.; Minowa, M.T.; Takeuchi, M.
    Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes (2006), Glycoconj. J., 23, 473-480.
    View publication on PubMed
Search for more literature for 2.4.1.145

Cloned(Commentary)

Cloned (Comment) Organism
recombinant full-length enzyme and soluble truncated form tagged with FLAG sequence at the N-terminus. expressed in COS7 cells, N-acetylglucosaminyltransferase-IVa Homo sapiens
recombinant full-length enzyme and soluble truncated form tagged with FLAG sequence at the N-terminus. expressed in COS7 cells, N-acetylglucosaminyltransferase-IVb Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.118
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVa Homo sapiens
0.242
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVb Homo sapiens
0.341
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 7.5, recombinant soluble human flag-GnTIVa Homo sapiens
0.358
-
 UDP-N-acetyl-D-glucosamine 37°C, pH 7.5, recombinant soluble human flag-GnTIVa Homo sapiens
0.532
-
 GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVa Homo sapiens
0.971
-
 GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVa Homo sapiens
1.04
-
 GlcNAcbeta1-2Man1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant soluble human flag-GnTIVa Homo sapiens
3.19
-
 GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVa Homo sapiens
3.35
-
 GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVb Homo sapiens
5.72
-
 GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVb Homo sapiens
6.94
-
 GlcNAcbeta1-2Man1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant soluble human flag-GnTIVa Homo sapiens
10.5
-
 GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine 37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVb Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens GnT-IVa is primarily responsible for the biosynthesis of complex-type N-glycans UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens GnT-IVb might contribute to the basal GnT-IV activity in cells UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R GnT-IVa is primarily responsible for the biosynthesis of complex-type N-glycans Homo sapiens UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R GnT-IVb might contribute to the basal GnT-IV activity in cells Homo sapiens UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVa Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVb Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVa Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVb Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVa Homo sapiens ?
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine N-acetylglucosaminyltransferase-IVb Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
GnT-IVb
-
 Homo sapiens
GnTIVa
-
 Homo sapiens
N-acetylglucosaminyltransferase-IVa
-
 Homo sapiens
N-acetylglucosaminyltransferase-IVb
-
 Homo sapiens