Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.144 extracted from

  • Allam, H.; Johnson, B.P.; Zhang, M.; Lu, Z.; Cannon, M.J.; Abbott, K.L.
    The glycosyltransferase GnT-III activates Notch signaling and drives stem cell expansion to promote the growth and invasion of ovarian cancer (2017), J. Biol. Chem., 292, 16351-16359 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.144

Application

Application Comment Organism
diagnostics GnT-III expression levels are elevated in high-grade serous ovarian carcinoma tissues and correlate with reduced survival Homo sapiens
medicine therapeutic potential of inhibiting GnT-III as a treatment for controlling epithelial ovarian carcinoma (EOC) growth and recurrence Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene MGAT3, quantitative real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information gene mgat3 suppression via shRNA. A significant increase in ovarian masses and metastasis in control shRNA cells compared with GnT-III shRNA cells is observed. Control cell tumors are more invasive following i.p. injections in NOD/SCID mice or human carcinoma samples, whereas GnT-III shRNA tumors are less vascularized and never invade surrounding tissues Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[Notch receptor] Homo sapiens
-
 UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[Notch receptor]
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] Homo sapiens
-
 UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q09327
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cancer stem cell
-
 Homo sapiens
-
additional information quantitative real-time PCR enzyme expression analysis Homo sapiens
-
ovary
-
 Homo sapiens
-
ovary cancer cell GnT-III expression significantly increases ovarian cancer growth and metastasis and increases the expansion of the ovarian cancer stem cell (CSC) population Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[Notch receptor]
-
 Homo sapiens UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[Notch receptor]
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
 Homo sapiens UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
-
 ?

Synonyms

Synonyms Comment Organism
GnT-III
-
 Homo sapiens
MGAT3
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction suppression of GnT-III in epithelial ovarian carcinoma (EOC) cell lines and primary tumor-derived cells results in an inhibition of Notch signaling that is more potent than pharmacologic blockage of Notch activation via gamma-secretase inhibition. The inhibition results from the redirection of the Notch receptor to the lysosome, a distinct mechanism Homo sapiens
physiological function glycosyltransferase GnT-III activates Notch signaling and drives stem cell expansion to promote the growth and invasion of ovarian cancer. Glycosylation mediated by GnT-III increases Notch receptor levels and activity. GnT-III expression controls the expansion of side-population cells, also known as cancer stem cells, GnT-III expression regulates the levels and activation of the heavily glycosylated Notch receptor involved in normal and malignant development. The gene Mgat3 encoding the glycosyltransferase GnT-III is elevated in epithelial ovarian carcinomas (EOCs) and leads to the production of abnormal truncated N-linked glycan structures instead of the typical bisected forms, role for bisecting glycosylation in the control of Notch transport, overview. GnT-III expression maintains the side-population and promotes spheroid formation in ovarian cancer cells Homo sapiens