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Literature summary for 2.4.1.144 extracted from

  • Link-Lenczowski, P.; Bubka, M.; Balog, C.I.A.; Koeleman, C.A.M.; Butters, T.D.; Wuhrer, M.; Litynska, A.
    The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells. GnT-III modifies highly branched N-glycans (2018), Glycoconj. J., 35, 217-231 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.144

Cloned(Commentary)

Cloned (Comment) Organism
gene MGAT3, stable overexpression of the enzyme in WM-266-4-GnT-III human metastatic melanoma cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information WM-266-4-GnT-III human metastatic melanoma cells stably overexpress the MGAT3 gene together with mock control cells WM-266-4-pIRESneo Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
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 Homo sapiens 5794
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] Homo sapiens
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 UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q09327
-
-

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell
-
 Homo sapiens
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melanoma cell
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 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 Homo sapiens UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 ?

Synonyms

Synonyms Comment Organism
GnT-III
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 Homo sapiens
MGAT3
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 Homo sapiens
N-acetylglucosaminyltransferase III
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 Homo sapiens

General Information

General Information Comment Organism
malfunction detailed glycomic analysis of the effect of GnT-III overexpression in WM266-4-GnT-III metastatic melanoma cells, overview. Overexpression of GnT-III in melanoma leads to modification of a broad range of N-glycan types by the introduction of the bisecting GlcNAc residue with highly branched complex structures among them. The presence of these unusual complex N-glycans results in stronger interactions of cellular glycoproteins with the PHA-L. Elevated activity of GnT-III in cancer cells does not necessarily lead to a total abrogation of the formation of highly branched glycans. The modification of pre-existing N-glycans by the introduction of bisecting GlcNAc can modulate their capacity to interact with carbohydrate-binding proteins such as plant lectins Homo sapiens
additional information usage of MALDI-TOF and ESIion-trap-MS/MS together with HILIC-HPLC of 2-AA labeled N-glycans to study the N-glycome of membrane-attached and secreted proteins. Characterization of N-glycan epitopes on membrane and secreted proteins using plant lectins, several plant lectins are used in lectin blot assay, overview Homo sapiens
physiological function N-acetylglucosaminyltransferase III (GnT-III) is known to catalyze N-glycan bisection and thereby modulate the formation of highly branched complex structures within the Golgi apparatus. While active, it inhibits the action of other GlcNAc transferases such as GnT-IVand GnT-V. Moreover, GnT-III is considered an inhibitor of the metastatic potential of cancer cells both in vitro and in vivo Homo sapiens