Cloned (Comment) | Organism |
---|---|
expression of soluble His-tagged protein in Spodoptera frugiperda Sf 21 insect cells via baculovirus infection, secretion into the medium | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
ADP-glucose | competitive against UDP-N-acetyl-D-glucosamine | Rattus norvegicus | |
alpha-N-acetyl-D-glucosamine-1-phosphate | - |
Rattus norvegicus | |
CDP | - |
Rattus norvegicus | |
CDP-glucose | competitive against UDP-N-acetyl-D-glucosamine | Rattus norvegicus | |
GDP | - |
Rattus norvegicus | |
GDP-glucose | competitive against UDP-N-acetyl-D-glucosamine | Rattus norvegicus | |
N-acetyl-D-glucosamine | - |
Rattus norvegicus | |
TDP | - |
Rattus norvegicus | |
TDP-glucose | competitive against UDP-N-acetyl-D-glucosamine | Rattus norvegicus | |
UDP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
pyridylaminated acceptor substrate | recombinant enzyme | Rattus norvegicus | |
0.021 | - |
pyridylaminated acceptor substrate | with UDP-GlcNAc as donor substrate | Rattus norvegicus | |
0.023 | - |
pyridylaminated acceptor substrate | recombinant enzyme | Rattus norvegicus | |
0.023 | - |
pyridylaminated acceptor substrate | with UDP-Glc as donor substrate | Rattus norvegicus | |
0.024 | - |
pyridylaminated acceptor substrate | recombinant enzyme | Rattus norvegicus | |
0.024 | - |
pyridylaminated acceptor substrate | with UDP-GalNAc as donor substrate | Rattus norvegicus | |
0.42 | - |
UDP-N-acetyl-D-glucosamine | recombinant enzyme | Rattus norvegicus | |
1 | - |
UDP-D-glucose | recombinant enzyme | Rattus norvegicus | |
3.6 | - |
UDP-N-acetyl-D-galactosamine | recombinant enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 60000 + x * 50000, SDS-PAGE | Rattus norvegicus |
60000 | - |
x * 60000 + x * 50000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from medium of Spodoptera frugiperda Sf 21 cell culture | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
purified recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UDP-galactose is no substrate | Rattus norvegicus | ? | - |
? | |
additional information | ADP-, CDP-, GDP-, and TDP-glucose are no donor substrates | Rattus norvegicus | ? | - |
? | |
UDP-D-glucose + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | pyridylaminated biantennary sugar chain | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-D-glucose + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | 0.2% activity compared to donor substrate UDP-N-acetyl-D-glucosamine | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-galactosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | pyridylaminated biantennary sugar chain | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-galactosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-galactosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | 0.1% activity compared to donor substrate UDP-N-acetyl-D-glucosamine | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-galactosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | pyridylaminated biantennary sugar chain | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + pyridylaminated acceptor substrate | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000 + x * 50000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-N-acetylglucosaminyltransferase III | - |
Rattus norvegicus |
GnT-III | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
ADP-glucose | recombinant enzyme | Rattus norvegicus | |
1 | - |
GDP-glucose | recombinant enzyme | Rattus norvegicus | |
3.9 | - |
CDP-glucose | recombinant enzyme | Rattus norvegicus |